卵泡液可增强人精子的吸引力及其活力。
Follicular fluid enhances sperm attraction and its motility in human.
作者信息
Jeon B G, Moon J S, Kim K C, Lee H J, Choe S Y, Rho G J
机构信息
Kaya Mother-Child's Hospital, Chinju, South Korea.
出版信息
J Assist Reprod Genet. 2001 Aug;18(8):407-12. doi: 10.1023/a:1016674302652.
PURPOSE
Follicular fluid has a pivotal effect on motility and chemotaxis of spermatozoa for successful fertilization. The effect of human follicular fluid (hFF) and progesterone on attraction and motility of spermatozoa were investigated using simplified capillary assays.
METHODS
Capillary tubes loaded with hFF, modified human tubal fluid (m-hTF), or m-hTF supplemented with progesterone, respectively, were used for assessments of attraction and motility of spermatozoa following culture at various time intervals.
RESULTS
Number and motile ratio of spermatozoa in the tubes loaded with hFF were significantly (P < .05) higher than those with m-hTF. In the tubes loaded with m-hTF, m-hTF supplemented with progesterone, and hFF, the attracted number of spermatozoa were 34 x 10(5), 131 x 10(5), and 108 x 10(5), and motile ratio of spermatozoa was 37, 48, and 82%, respectively.
CONCLUSIONS
We conclude that hFF clearly plays a crucial role in enhancing attraction and motility of spermatozoa, and progesterone has strong effect on attraction of spermatozoa.
目的
卵泡液对精子的运动能力和趋化性具有关键作用，这对于成功受精至关重要。本研究采用简化的毛细管试验，探究人卵泡液（hFF）和孕酮对精子吸引和运动能力的影响。
方法
分别将装有hFF、改良人输卵管液（m-hTF）或添加孕酮的m-hTF的毛细管用于评估精子在不同培养时间间隔后的吸引和运动能力。
结果
装有hFF的毛细管中的精子数量和活动率显著高于装有m-hTF的毛细管（P < 0.05）。在装有m-hTF、添加孕酮的m-hTF和hFF的毛细管中，吸引的精子数量分别为34×10⁵、131×10⁵和108×10⁵，精子活动率分别为37%、48%和82%。
结论
我们得出结论，hFF在增强精子吸引和运动能力方面显然起着关键作用，而孕酮对精子吸引具有强烈影响。
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