Li Yue, Liu Hui, Yu Qingqing, Liu Hongbin, Huang Tao, Zhao Shigang, Ma Jinlong, Zhao Han
Center for Reproductive Medicine, Shandong University, Jinan, China.
The Key Laboratory of Reproductive Endocrinology of Ministry of Education, Jinan, China.
Front Endocrinol (Lausanne). 2019 Jul 24;10:485. doi: 10.3389/fendo.2019.00485. eCollection 2019.
Increasing the success rate of maturation (IVM) for human oocytes has a major clinical significance. Previous studies have shown that growth hormone (GH) added into IVM medium could promote IVM of oocytes from non-human beings. However, few studies on systematic IVM for human oocytes with GH have been reported. Human germinal vesicle (GV) oocytes collected for IVM were cultured with different concentrations of GH to optimize the concentration. Metaphase II (MII) stage oocytes obtained from IVM were fertilized by intracytoplasmic sperm injection (ICSI). Maturation rate, fertilization rate, and blastocyst rate were assessed after IVM with or without GH. Furthermore, gene expression profiles were compared in oocytes between the two groups using single-cell RNA-seq. The optimal concentration of GH for IVM was 200 ng/ml, and the maturation rate of this group reached 70% which was double that of the control group (35%, = 0.004). The fertilization rate (73.1 vs. 60.3%) and blastocyst rate (25.0 vs. 15.5%) both had an increasing trend in the GH group compared to controls. Single-cell RNA-Seq and real-time PCR data showed that GH could significantly enhance the expression of genes associated with meiotic progression and embryo development, such as (aurora kinase A, = 0.007), (protein disulfide isomerase family A member 6, = 0.007), (leucine rich repeat and Ig domain containing 2, = 0.007), and (centromere protein J, = 0.039). Taken together, GH could promote maturation of human oocytes, probably through accelerating meiotic progression, balancing redox homeostasis of cellular environment, and promoting oocyte developmental competence.
提高人类卵母细胞的成熟率（IVM）具有重大临床意义。先前的研究表明，添加到IVM培养基中的生长激素（GH）可促进非人动物卵母细胞的IVM。然而，关于使用GH对人类卵母细胞进行系统性IVM的研究报道较少。收集用于IVM的人类生发泡（GV）卵母细胞，用不同浓度的GH进行培养以优化浓度。从IVM获得的中期II（MII）期卵母细胞通过胞浆内单精子注射（ICSI）进行受精。在有或没有GH的IVM后评估成熟率、受精率和囊胚率。此外，使用单细胞RNA测序比较两组卵母细胞的基因表达谱。IVM的最佳GH浓度为200 ng/ml，该组的成熟率达到70%，是对照组（35%， = 0.004）的两倍。与对照组相比，GH组的受精率（73.1%对60.3%）和囊胚率（25.0%对15.5%）均呈上升趋势。单细胞RNA测序和实时PCR数据表明，GH可显著增强与减数分裂进程和胚胎发育相关基因的表达，如 （极光激酶A， = 0.007）、 （蛋白质二硫键异构酶家族A成员6， = 0.007）、 （富含亮氨酸重复序列和免疫球蛋白结构域的蛋白2， = 0.007）和 （着丝粒蛋白J， = 0.039）。综上所述，GH可能通过加速减数分裂进程、平衡细胞环境的氧化还原稳态以及提高卵母细胞发育能力来促进人类卵母细胞的成熟。
