Agarwal M, Katiyar-Agarwal S, Sahi C, Gallie D R, Grover A
Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, India.
Cell Stress Chaperones. 2001 Jul;6(3):219-24. doi: 10.1379/1466-1268(2001)006<0219:athpka>2.0.co;2.
Arabidopsis thaliana, the first plant for which the entire genome sequence is available, was also among the first plant species from which Hsp100 proteins were characterized. The Athsp101 complementary DNA (cDNA) corresponds to the gene identification At1g74310 in the Arabidopsis genome sequence. Analysis of the genome revealed 7 additional proteins that are variably homologous with At1g74310 throughout the entire amino acid sequence and significant similarities or identities in the signature sequences conserved among Hsp100 proteins. Although AtHsp101 is cytoplasmic, 5 of the 7 related proteins have predicted plastidial localization signals. This complete description of the AtHsp100 family sets the stage for future research on expression and function.
拟南芥是第一个拥有完整基因组序列的植物，也是最早对热激蛋白100（Hsp100）进行蛋白特性鉴定的植物物种之一。拟南芥热激蛋白101（Athsp101）互补DNA（cDNA）与拟南芥基因组序列中的基因标识At1g74310相对应。基因组分析显示，还有另外7种蛋白质在整个氨基酸序列上与At1g74310存在不同程度的同源性，并且在热激蛋白100保守的特征序列上具有显著的相似性或一致性。尽管AtHsp101存在于细胞质中，但这7种相关蛋白中有5种具有预测的质体定位信号。对AtHsp100家族的这一完整描述为未来关于其表达和功能的研究奠定了基础。
