Kissen Ralph, Øverby Anders, Winge Per, Bones Atle M
Department of Biology, Norwegian University of Science and Technology (NTNU), NO-7491, Trondheim, Norway.
Present address: Center for Clinical Pharmacy and Clinical Sciences, School of Pharmaceutical Sciences, Kitasato University, Minato-ku, Tokyo, Japan.
BMC Genomics. 2016 Sep 17;17(1):740. doi: 10.1186/s12864-016-3039-x.
Isothiocyanates (ITCs) are degradation products of the plant secondary metabolites glucosinolates (GSLs) and are known to affect human health as well as plant herbivores and pathogens. To investigate the processes engaged in plants upon exposure to isothiocyanate we performed a genome scale transcriptional profiling of Arabidopsis thaliana at different time points in response to an exogenous treatment with allyl-isothiocyanate.
The treatment triggered a substantial response with the expression of 431 genes affected (P < 0.05 and log2 ≥ 1 or ≤ -1) already after 30 min and that of 3915 genes affected after 9 h of exposure, most of the affected genes being upregulated. These are involved in a considerable number of different biological processes, some of which are described in detail: glucosinolate metabolism, sulphate uptake and assimilation, heat stress response, oxidative stress response, elicitor perception, plant defence and cell death mechanisms.
Exposure of Arabidopsis thaliana to vapours of allyl-isothiocyanate triggered a rapid and substantial transcriptional response affecting numerous biological processes. These include multiple stress stimuli such as heat stress response and oxidative stress response, cell death and sulphur secondary defence metabolism. Hence, effects of isothiocyanates on plants previously reported in the literature were found to be regulated at the gene expression level. This opens some avenues for further investigations to decipher the molecular mechanisms underlying the effects of isothiocyanates on plants.
异硫氰酸盐（ITCs）是植物次生代谢产物硫代葡萄糖苷（GSLs）的降解产物，已知其会影响人类健康以及植物食草动物和病原体。为了研究植物在接触异硫氰酸盐时所涉及的过程，我们对拟南芥在不同时间点对外源烯丙基异硫氰酸盐处理的反应进行了全基因组转录谱分析。
处理引发了显著反应，30分钟后已有431个基因的表达受到影响（P < 0.05且log2≥1或≤ -1），暴露9小时后有3915个基因的表达受到影响，大多数受影响的基因被上调。这些基因参与了大量不同的生物学过程，其中一些将详细描述：硫代葡萄糖苷代谢、硫酸盐吸收和同化、热应激反应、氧化应激反应、激发子感知、植物防御和细胞死亡机制。
拟南芥暴露于烯丙基异硫氰酸盐蒸汽中引发了快速且显著的转录反应，影响了众多生物学过程。这些过程包括多种应激刺激，如热应激反应和氧化应激反应、细胞死亡以及硫次生防御代谢。因此，文献中先前报道的异硫氰酸盐对植物的影响在基因表达水平上受到调控。这为进一步研究揭示异硫氰酸盐对植物影响的分子机制开辟了一些途径。
