Miernyk J A
Plant Genetics Research Unit, US Department of Agriculture, Agricultural Research Service, University of Missouri, Columbia 65211, USA.
Cell Stress Chaperones. 2001 Jul;6(3):209-18. doi: 10.1379/1466-1268(2001)006<0209:tjdpoa>2.0.co;2.
A total of 89 J-domain proteins were identified in the genome of the model flowering plant Arabidopsis thaliana. The deduced amino acid sequences of the J-domain proteins were analyzed for an assortment of structural features and motifs. Based on the results of sequence comparisons and structure and function predictions, 51 distinct families were identified. The families ranged in size from 1 to 6 members. Subcellular localizations of the A thaliana J-domain proteins were predicted; species were found in both the soluble and membrane compartments of all cellular organelles. Based on digital Northern analysis, the J-domain proteins could be separated into groups of low, medium, and moderate expression levels. This genomics-based analysis of the A thaliana J-domain proteins establishes a framework for detailed studies of biological function and specificity. It additionally provides a comprehensive basis for evolutionary comparisons.
在模式开花植物拟南芥的基因组中总共鉴定出89种J结构域蛋白。对这些J结构域蛋白推导的氨基酸序列进行了各种结构特征和基序分析。基于序列比较以及结构和功能预测的结果，鉴定出了51个不同的家族。这些家族的大小从1个成员到6个成员不等。预测了拟南芥J结构域蛋白的亚细胞定位；发现这些蛋白存在于所有细胞器的可溶性和膜性区室中。基于数字Northern分析，J结构域蛋白可分为低、中、中等表达水平的组。这种基于基因组学对拟南芥J结构域蛋白的分析为详细研究生物学功能和特异性建立了框架。它还为进化比较提供了全面的基础。
