通过聚合酶链反应（PCR）和原位杂交法测定的异位妊娠或输卵管因素不孕症患者新鲜组织标本中沙眼衣原体的患病率。
The prevalence of Chlamydia trachomatis in fresh tissue specimens from patients with ectopic pregnancy or tubal factor infertility as determined by PCR and in-situ hybridisation.
作者信息
Barlow Rachael E L, Cooke Ian D, Odukoya Olusegun, Heatley Mark K, Jenkins Julian, Narayansingh Gordon, Ramsewak Samuel S, Eley Adrian
机构信息
Division of Genomic Medicine, University of Sheffield Medical School, Floor F, Sheffield S10 2RX, *Department of Obstetrics and Gynaecology, Divison of Surgical and Anaesthetic Sciences, University of Sheffield Medical School, Sheffield, †Department of Pathology, Royal Liverpool and Broadgreen University Hospitals NHS Trust, Liverpool, ‡Division of Obstetrics and Gynaecology, Department of Hospital Medicine, University of Bristol, Bristol, UK and §Department of Clinical Surgical Sciences, University of the West Indies, St Augustine, Trinidad.
出版信息
J Med Microbiol. 2001 Oct;50(10):902-908. doi: 10.1099/0022-1317-50-10-902.
The prevalence of chlamydial DNA determined by PCR and in-situ hybridisation (ISH) in fresh tissue specimens (endometrium, fallopian tube and ovary) was investigated in 33 women presenting with ectopic pregnancy (EP), 14 women with tubal factor infertility (TFI) and 50 control patients from the UK and the West Indies. In the UK EP group, chlamydial DNA was detected by PCR in 56% of patients; similar results were found in the Trinidad EP group (67%). In the TFI group, chlamydial DNA was detected in (71%) of patients by PCR. The detection of Chlamydia trachomatis DNA by ISH was highest in the TFI group (43%). Women presenting with EP and TFI showed evidence of previous or current genital C. trachomatis infection, underlining the importance of this microorganism in the development of these conditions. Importantly, chlamydial DNA could be detected in DNA preparations from the endometrium, fallopian tube and ovary of EP and TFI patients at the time of surgery.
我们对33例宫外孕（EP）患者、14例输卵管因素不孕（TFI）患者以及50例来自英国和西印度群岛的对照患者的新鲜组织标本（子宫内膜、输卵管和卵巢）进行研究，通过聚合酶链反应（PCR）和原位杂交（ISH）来检测衣原体DNA的流行情况。在英国的EP组中，通过PCR检测发现56%的患者存在衣原体DNA；在特立尼达的EP组中也发现了类似结果（67%）。在TFI组中，通过PCR检测发现71%的患者存在衣原体DNA。通过ISH检测沙眼衣原体DNA的比例在TFI组中最高（43%）。患有EP和TFI的女性显示出既往或当前生殖道沙眼衣原体感染的证据，这突出了这种微生物在这些病症发展中的重要性。重要的是，在手术时，可从EP和TFI患者的子宫内膜、输卵管和卵巢的DNA制剂中检测到衣原体DNA。
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