Hajikhani Bahareh, Motallebi Tayebeh, Norouzi Jamileh, Bahador Abbas, Bagheri Rezvan, Asgari Soheila, Chamani-Tabriz Leili
Reproductive Infections Department of Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
J Reprod Infertil. 2013 Jan;14(1):29-33.
Chlamydia trachomatis is the most reported bacterial sexually transmitted disease, especially among young women worldwide. The aim of this study was comparison the prevalence of Chlamydia trachomatis infection in woman with tubal infertility by means of PCR and cell culture techniques.
Fifty-one women with confirmed TFI were enrolled in this study in (avicenna infertility Clinic) between January 2010 and January 2011. Cervical swab and cytobrush specimens were collected from each patient by gynecologists and sent to laboratory in transport media. Detection of Chlamydia trachomatis in samples was performed using PCR and bacteria culture in MacCoy cell line. The data were analyzed by Fisher's exact test and independent t-test. Statistical significance was established at a p-value <0.05.
A significant relation was observed between increased the age of first intercourse and chlamydial infection. Six (11.7%) samples had positive PCR result, whereas cell culture results were positive in only 2 (3.9%) samples. A significant relation was also identified between the duration of infertility and infection (p < 0.05) by PCR versus cell culture method.
The results showed that PCR is a rapid method, compared to cell culture for detecting Chlamydial organism. It also became clear that the age at first intercourse is important to predict the likelihood of Chlamydia trachomatis.
沙眼衣原体是报告最多的细菌性性传播疾病，在全球年轻女性中尤为常见。本研究的目的是通过聚合酶链反应（PCR）和细胞培养技术比较输卵管性不孕女性中沙眼衣原体感染的患病率。
2010年1月至2011年1月期间，51名确诊为输卵管性不孕（TFI）的女性纳入本研究（在阿维森纳不孕诊所）。妇科医生从每位患者采集宫颈拭子和细胞刷标本，并在转运培养基中送至实验室。使用PCR和在 McCoy 细胞系中进行细菌培养来检测样本中的沙眼衣原体。数据采用 Fisher 精确检验和独立 t 检验进行分析。p 值<0.05 时确定具有统计学意义。
首次性交年龄增加与衣原体感染之间存在显著关联。6份（11.7%）样本PCR结果为阳性，而细胞培养结果仅2份（3.9%）样本为阳性。通过PCR与细胞培养方法还发现不孕持续时间与感染之间存在显著关联（p<0.05）。
结果表明，与细胞培养相比，PCR是检测衣原体病原体的快速方法。还明确了首次性交年龄对于预测沙眼衣原体感染可能性很重要。
