Borges M H, Soares A M, Rodrigues V M, Oliveira F, Fransheschi A M, Rucavado A, Giglio J R, Homsi-Brandeburgo M I
Departamento de Genética e Bioquímica, Universidade Federal de Uberlândia, UFU, 38400-902 Uberlândia, MG, Brazil.
Toxicon. 2001 Dec;39(12):1863-9. doi: 10.1016/s0041-0101(01)00169-6.
Aqueous extract from Casearia sylvestris leaves, a typical plant from Brazilian open pastures, was able to neutralize the hemorrhagic activity caused by Bothrops asper, Bothrops jararacussu, Bothrops moojeni, Bothrops neuwiedi and Bothrops pirajai venoms. It also neutralized two hemorrhagic metalloproteinases from Bothrops asper venom. Proteolytic activity on casein induced by bothropic venoms and by isolated proteases, including Bn2 metalloproteinase from B. neuwiedi venom, was also inhibited by the C. sylvestris extract in different levels. The alpha-fibrinogen chain was partially protected against degradation caused by B. jararacussu venom, when this venom was incubated with C. sylvestris extract. We also observed that this extract partially increased the time of plasma coagulation caused by B. jararacussu, B. moojeni and B. neuwiedi venoms. C. sylvestris extract did not induce proteolysis in any substrate assayed.
来自巴西开阔牧场的典型植物——野鸦椿（Casearia sylvestris）叶片的水提取物，能够中和由矛头蝮（Bothrops asper）、巴西矛头蝮（Bothrops jararacussu）、莫氏矛头蝮（Bothrops moojeni）、纳氏矛头蝮（Bothrops neuwiedi）和皮氏矛头蝮（Bothrops pirajai）毒液引起的出血活性。它还能中和来自矛头蝮毒液的两种出血性金属蛋白酶。野鸦椿提取物也能不同程度地抑制由蝰蛇毒液和包括来自纳氏矛头蝮毒液的Bn2金属蛋白酶在内的分离蛋白酶诱导的酪蛋白水解活性。当巴西矛头蝮毒液与野鸦椿提取物一起孵育时，α-纤维蛋白原链受到部分保护，免受巴西矛头蝮毒液引起的降解。我们还观察到，这种提取物能部分延长由巴西矛头蝮、莫氏矛头蝮和纳氏矛头蝮毒液引起的血浆凝固时间。野鸦椿提取物在任何检测的底物中均未诱导蛋白水解。
