Prakash M, Kapembwa M S, Gotch F, Patterson S
Department of Immunology, Imperial College of Science Technology and Medicine (ICSTM) at Chelsea and Westminster Hospital, 369 Fulham Road, London, UK.
J Reprod Immunol. 2001 Oct-Nov;52(1-2):101-11. doi: 10.1016/s0165-0378(01)00114-0.
Heterosexual transmission of human immunodeficiency virus (HIV-1) is the predominant mode of infection world-wide. To better understand sexual transmission of HIV-1 in women we have analysed virus co-receptor and cellular activation marker expression on T lymphocyte subsets from the cervical epithelium and have made comparisons with peripheral blood T cells. Intraepithelial cervical T lymphocytes were obtained with a cytobrush, immunolabelled and analysed by flow cytometry. Activation markers (CD69, CD25 and HLA-DR) were found to be more highly expressed on cervical than on blood T lymphocytes. These higher levels of activation on cervical T lymphocyte subsets could facilitate HIV-1 infection. CXCR4 was expressed at marginally higher levels than CCR5 on T cells from the cervical epithelium and peripheral blood. Thus, the preferential transmission of macrophage tropic strains of HIV-1 following sexual contact cannot be explained solely on the expression of chemokine co-receptors by T lymphocyte subsets.
人类免疫缺陷病毒（HIV-1）的异性传播是全球主要的感染方式。为了更好地了解HIV-1在女性中的性传播情况，我们分析了来自宫颈上皮的T淋巴细胞亚群上病毒共受体和细胞活化标志物的表达，并与外周血T细胞进行了比较。用细胞刷获取宫颈上皮内T淋巴细胞，进行免疫标记并通过流式细胞术分析。发现活化标志物（CD69、CD25和HLA-DR）在宫颈T淋巴细胞上的表达高于血液T淋巴细胞。宫颈T淋巴细胞亚群上这些更高水平的活化可能会促进HIV-1感染。CXCR4在宫颈上皮和外周血T细胞上的表达略高于CCR5。因此，性接触后HIV-1巨噬细胞嗜性毒株的优先传播不能仅用T淋巴细胞亚群上趋化因子共受体的表达来解释。
