反相高效液相色谱法测定当药中环烯醚萜类和三萜类成分

[Determination of iridoids and triterpenes in herb of Swertia pseudochinesis by RP-HPLC].

作者信息

Huang Wen-Zhe, Xu Chun-Hui, Zhou Da-Cheng, Tong Li-Hua, Ye Zu-Guang

机构信息

State Research Center for R&D of TCM Multi-ingredient Drugs, Beijing 100075, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2007 Dec;32(23):2494-6.

PMID:18330241

Abstract

OBJECTIVE

To establish a RP-HPLC method for the determination of swertiamarin, sweroside, gentiopicrin and oleanolic acid in different parts of Swertia pseudochinesis.

METHOD

A Zorbax SB-C18 (4.6 mm x 250 mm, 5 microm) column was used with acetonitrile-water (10:90) and methnol-water(86:14) at detection wavelengths of 238 nm, 246 nm, 274 nm and 207 nm for swertiamarin, sweroside, gentiopicrin and oleanolic acid respectively. The flow rate was 1.0 mL x min(-1) and the column temperature was 25 degrees C.

RESULT

All of the compounds were based--isolated. The linear ranges of swertiamarin, sweroside, gentiopicrin and oleanolic acid were 0.068 9-0.344 4(r = 0.999 2) , 0.001 1-0.014 0 (r2 = 0. 999 8), 0.001 1-0.013 4 (r2 = 0.999 9) and 0.001 1-0.008 8 mg x mL(-1) (r2 = 0. 999 6), respectively.

CONCLUSION

The method is simple and accurate, which can be used for quality control of S. pseudochinesis.

摘要

目的

建立一种反相高效液相色谱法（RP-HPLC），用于测定当药不同部位中獐牙菜苦苷、獐牙菜苷、龙胆苦苷和齐墩果酸的含量。

方法

采用Zorbax SB-C18（4.6 mm×250 mm，5μm）色谱柱，以乙腈-水（10:90）和甲醇-水（86:14）为流动相，检测波长分别为238 nm、246 nm、274 nm和207 nm，用于测定獐牙菜苦苷、獐牙菜苷、龙胆苦苷和齐墩果酸。流速为1.0 mL·min⁻¹，柱温为25℃。

结果

所有化合物均得到基线分离。獐牙菜苦苷、獐牙菜苷、龙胆苦苷和齐墩果酸的线性范围分别为0.068 9 - 0.344 4（r = 0.999 2）、0.001 1 - 0.014 0（r² = 0.999 8）、0.001 1 - 0.013 4（r² = 0.999 9）和0.001 1 - 0.008 8 mg·mL⁻¹（r² = 0.999 6）。