Martí J, Wills K V, Ghetti B, Bayer S A
Departament de Biologia Cel.lular, de Fisiologia i d'Immunologia, Unitat de Citologia i d'Histologia, Facultat de Ciències, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
Int J Dev Neurosci. 2001 Oct;19(6):599-610. doi: 10.1016/s0736-5748(01)00036-3.
To determine whether the neurogenetic patterns of Purkinje cells and deep cerebellar nuclei neurons were normal in weaver homozygotes and whether the degeneration of those neuronal types was linked to their time of origin, [3H] thymidine autoradiography was applied on sections of homozygous weaver mice and normal controls on postnatal day 90. The experimental animals were the offspring of pregnant dams injected with [3H] thymidine on embryonic days 11-12, 12-13, 13-14 and 14-15. The results show that the onset of neurogenesis, its pattern of peaks and valleys, and its total span were similar between wild type and homozygous weaver in the cerebellar areas analyzed, indicating that the loss of Purkinje cells and deep cerebellar nuclei neurons is not related to neurogenetic patterns. In weaver homozygotes, the loss of Purkinje cells and deep cerebellar nuclei neurons followed a lateral to medial gradient of increasing severity. Thus, the vermis and the fastigial nucleus, which are medially located, presented the most important neuron loss, whereas in the lateral hemisphere and the dentate nucleus, neuron loss was spared.
为了确定织工纯合子中浦肯野细胞和小脑深部核团神经元的神经发生模式是否正常，以及这些神经元类型的退化是否与其起源时间有关，在出生后第90天对纯合子织工小鼠和正常对照的切片进行了[3H]胸腺嘧啶核苷放射自显影。实验动物是在胚胎第11 - 12天、12 - 13天、13 - 14天和14 - 15天注射[3H]胸腺嘧啶核苷的怀孕母鼠的后代。结果表明，在所分析的小脑区域中，野生型和纯合子织工小鼠的神经发生起始、其峰谷模式及其总跨度相似，这表明浦肯野细胞和小脑深部核团神经元的丧失与神经发生模式无关。在织工纯合子中，浦肯野细胞和小脑深部核团神经元的丧失遵循从外侧到内侧严重程度增加的梯度。因此，位于内侧的蚓部和顶核神经元丧失最为严重，而外侧半球和齿状核的神经元丧失较少。
