Gould B B, Rakic P
Exp Brain Res. 1981;44(2):195-206. doi: 10.1007/BF00237341.
The genesis of the neurons that form the cerebellar nuclei was studied by autoradiographic methods in 30 postnatal rhesus monkeys which were exposed to 3H-thymidine at various embryonic (E) and postnatal (P) ages. As a basis for this quantitative analysis, five 2-3 month old monkeys were used for cell counting and estimation of the total number of neurons in each of the cerebellar nuclei. The results show that the cerebellar nuclei on each side contain 131,000 neurons. There are 68,000 neurons in the dentate nucleus, 25,000 neurons in the posterior interposed nucleus, and 19,000 neurons in both the anterior interposed the fastigial nuclei. All of the neurons comprising the deep nuclei are generated during the first half of the 165 days gestation period in this species. Although neurogenesis lasts from E30 through E70, approximately 81% of the neuron population is generated during a one week period between E36 and E40, with the peak of proliferation occurring at E36. Before E45 both large (maximum diameter greater than 35 micrometers) and small (maximum diameter 35 micrometers or less) neurons are produced simultaneously; after this period only small neurons are generated. Although no clearcut spatio-temporal gradients of neurogenesis could be discerned along any of the cardinal axes, each cerebellar nucleus has a somewhat distinctive developmental history in terms of the onset and cessation of neurogenesis and the tempo of cell proliferation. Thus, genesis of neurons destined for the dentate nucleus begins earlier and ends later than proliferation of the neurons that ultimately comprise the fastigial nucleus. Generation of the neurons destined for the anterior and posterior interposed nuclei follows an intermediate time course. The present data on neurogenetic sequences in the deep nuclei could not be correlated with the zonal pattern of reciprocal axonal connections that link the deep nuclei and overlying cerebellar cortex.
采用放射自显影方法，对30只出生后的恒河猴进行研究，这些猴子在不同胚胎期（E）和出生后（P）阶段暴露于3H-胸腺嘧啶。作为该定量分析的基础，选用了5只2 - 3个月大的猴子进行细胞计数，并估算每个小脑核中神经元的总数。结果显示，每侧小脑核含有131,000个神经元。齿状核中有68,000个神经元，后间位核中有25,000个神经元，前间位核和顶核中各有19,000个神经元。构成深部核团的所有神经元均在该物种165天妊娠期的前半段产生。虽然神经发生从E30持续到E70，但约81%的神经元群体是在E36至E40的一周内产生的，增殖高峰出现在E36。在E45之前，大神经元（最大直径大于35微米）和小神经元（最大直径35微米或更小）同时产生；在此之后只产生小神经元。尽管沿任何主轴线都无法辨别出明确的神经发生时空梯度，但就神经发生的起始和终止以及细胞增殖的节奏而言，每个小脑核都有 somewhat distinctive的发育史。因此，注定进入齿状核的神经元的发生开始得更早，结束得更晚，比最终构成顶核的神经元的增殖要晚。注定进入前间位核和后间位核的神经元的产生遵循中间的时间进程。目前关于深部核团神经发生序列的数据与连接深部核团和上方小脑皮质的相互轴突连接的带状模式无法相关联。 （注：原文中“somewhat distinctive”直译为“有点独特的”，这里根据语境意译为“有所不同的”更通顺，但按要求需保留原文。）
