Martí Joaquín, Santa-Cruz M C, Hervás José P
Unidad de Citología e Histología, Facultad de Biociencias, Universidad Autónoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
Unidad de Citología e Histología, Facultad de Biociencias, Universidad Autónoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
Int J Dev Neurosci. 2016 Apr;49:37-45. doi: 10.1016/j.ijdevneu.2015.12.006. Epub 2015 Dec 31.
Production and death of deep cerebellar nuclei (DCN) neurons were investigated in the weaver condition at appropriate anatomical levels throughout the mediolateral (medial, intermediate and lateral) and rostrocaudal (rostral, middle and caudal) axes of three DCN-cell groups: the fastigial, the interposed and the dentate nuclei. Current results have denoted that the deficit of DCN neurons is always more important in the homozygous weaver than in the heterozygous weaver mice. No loss of neurons was found in the dentate nucleus. In the mediolateral axis, an intranuclear gradient of depletion was observed in the mutant mice; in a given deep nucleus, neurodegeneration was more prominent in the medial pars than in lateral ones. In the rostrocaudal axis, on the other hand, when each deep nucleus was studied and compared as a whole, neuron loss was higher in the fastigial nucleus than in the interposed nucleus, which, in turn, was more important than in the dentate nucleus. These data suggest that, in the weaver condition, an internuclear gradient of neurodegeneration exists. Moreover, neurons located in rostral parts of a given nucleus appear to be more vulnerable than those settled in middle parts and these, in turn, are more than the caudal ones. These results seem to indicate the presence of an intranuclear gradient of depletion. Current autoradiographic results have revealed that, in the rostrocaudal axis, deep neurons are settled in the weaver cerebellum following three neurogenetic gradients. The first of these is internuclear; if each deep nucleus is analyzed and compared as a whole, the fastigial nucleus has more late-generated neurons than the interposed nucleus, and this, in turn, has more than the dentate nucleus. The second gradient is also internuclear; if the proportion of late-born neurons is compared throughout the rostral levels from each deep nucleus, it is observed that proportions increase from the fastigial to the dentate nucleus. A similar picture emerges when the middle and caudal regions are taken into account. The third gradient is intranuclear; in a given deep nucleus, the rostral region always presents more late-produced neurons than the middle region and these, in turn, more than in the caudal level.
在织工小鼠模型中，研究了小脑深部核团（DCN）神经元在整个三个DCN细胞群（顶核、间位核和齿状核）的内外侧（内侧、中间和外侧）以及前后轴（嘴侧、中间和尾侧）的适当解剖水平上的产生和死亡情况。目前的结果表明，纯合织工小鼠中DCN神经元的缺陷总是比杂合织工小鼠中的更严重。在齿状核中未发现神经元丢失。在内外侧轴上，在突变小鼠中观察到核内的神经元缺失梯度；在给定的深部核团中，内侧部分的神经退行性变比外侧部分更明显。另一方面，在前后轴上，当将每个深部核团作为一个整体进行研究和比较时，顶核中的神经元丢失比间位核中的更高，而间位核中的神经元丢失又比齿状核中的更严重。这些数据表明，在织工小鼠模型中，存在神经退行性变的核间梯度。此外，位于给定核团嘴侧部分的神经元似乎比位于中间部分的神经元更易受损，而中间部分的神经元又比尾侧部分的更易受损。这些结果似乎表明存在核内的神经元缺失梯度。目前的放射自显影结果显示，在前后轴上，深部神经元在织工小鼠小脑中的分布遵循三个神经发生梯度。其中第一个是核间梯度；如果将每个深部核团作为一个整体进行分析和比较，顶核中晚期产生的神经元比间位核中的更多，而间位核中的又比齿状核中的更多。第二个梯度也是核间梯度；如果比较每个深部核团在整个嘴侧水平上晚期出生神经元的比例，会观察到从顶核到齿状核比例逐渐增加。当考虑中间和尾侧区域时，也会出现类似的情况。第三个梯度是核内梯度；在给定的深部核团中，嘴侧区域总是比中间区域有更多晚期产生的神经元，而中间区域又比尾侧水平的更多。
