Sait Keles M, Taysi S, Aksoy H, Sen N, Polat F, Akcay F
Department of Biochemistry, Faculty of Medicine, Ataturk University, Erzurum, Turkey.
Clin Chem Lab Med. 2001 Sep;39(9):827-9. doi: 10.1515/CCLM.2001.137.
Multiple sclerosis is a disease characterized by perivascular infiltrates and demyelination of the white matter in the central nervous system. In this study, we compared the serum and cerebrospinal fluid nitric oxide levels before and after methylprednisolone therapy, and during remission period, and investigated the relationship of nitric oxide to the activity of multiple sclerosis. Cerebrospinal fluid and serum nitric oxide levels were measured blind as nitrite plus nitrate, using the nitrate reductase and Griess reaction method in 20 patients with multiple sclerosis before and after corticosteroid therapy, and during remission period, and in 20 control subjects. Mean cerebrospinal fluid and serum nitric oxide levels were highest in the pretreatment group and lowest in the control group. There was no correlation with nitric oxide levels in these two groups. Although corticosteroid therapy did not have any great effect on Expanded Disability Status Scales, it led to a decrease in nitric oxide levels. The possible cause of this might be the inhibition of nitric oxide synthesis by methylprednisolone, or a decrease in multiple sclerosis activity. We conclude that serum or cerebrospinal fluid nitric oxide levels do not reflect the activity in multiple sclerosis.
多发性硬化症是一种以中枢神经系统白质血管周围浸润和脱髓鞘为特征的疾病。在本研究中，我们比较了甲基强的松龙治疗前后、缓解期血清和脑脊液中一氧化氮水平，并研究了一氧化氮与多发性硬化症活动度之间的关系。采用硝酸还原酶和格里斯反应法，对20例多发性硬化症患者在皮质类固醇治疗前后、缓解期以及20例对照者的脑脊液和血清一氧化氮水平进行盲法测定，以亚硝酸盐加硝酸盐的形式表示。预处理组的脑脊液和血清一氧化氮平均水平最高，对照组最低。这两组的一氧化氮水平之间无相关性。虽然皮质类固醇治疗对扩展残疾状态量表没有太大影响，但它导致了一氧化氮水平的降低。其可能原因可能是甲基强的松龙抑制了一氧化氮的合成，或者是多发性硬化症活动度降低。我们得出结论，血清或脑脊液一氧化氮水平不能反映多发性硬化症的活动度。
