Department of Medical Laboratory Science, Faculty of Health Sciences and Technology, University of Nigeria Enugu Campus, Enugu 200241, Nigeria.
Centre National de Référence des Hépatites B, C et Delta, Laboratoire de Microbiologie Clinique, Hôpital-Avicenne, Assistance Publique Hôpitaux de Paris, Université Sorbonne Paris Cité, 93000 Bobigny, France.
Viruses. 2024 Jul 31;16(8):1236. doi: 10.3390/v16081236.
Hepatitis delta virus (HDV) is a satellite of hepatitis B virus (HBV), which requires the HBV surface antigen (HBsAg) for its assembly and propagation. Although countries affected by HBV infection in Africa are well identified, data on HDV infection are still scarce, like in Nigeria, where HBV infection is endemic. In this study, we aimed to determine the prevalence of HDV infection and identify the circulating genotypes/strains in the country. A nationwide study was performed on 1281 HBsAg-positive samples collected from patients across eleven sites drawn from the six geopolitical zones in Nigeria. Anti-HDV antibody (HDV-Ab) screening and HDV-RNA viral load quantification were performed using a commercial ELISA assay and real-time RT-PCR kit, respectively. HDV genotyping was performed by the Sanger sequencing of amplicons from the so-called region of the viral genome, followed by phylogenetic analyses. Of the 1281 HBsAg-positive samples, 61 (4.8%) were HDV-Ab positive, among which, 12 (19.7%) were HDV-RNA positive. Genotypes were obtained for nine of them: seven "African" HDV-1, one "Asian/European" HDV-1 and one HDV-6. This study shows that Nigeria is a country of low HDV prevalence where mainly "African" genotype-1 strains are circulating.
丁型肝炎病毒 (HDV) 是乙型肝炎病毒 (HBV) 的卫星病毒，其组装和繁殖需要 HBV 表面抗原 (HBsAg)。尽管非洲受 HBV 感染的国家已经得到很好的确定，但有关 HDV 感染的数据仍然很少，例如在尼日利亚，HBV 感染是地方性的。在这项研究中，我们旨在确定 HDV 感染的流行率，并确定该国的循环基因型/株。在尼日利亚的 11 个地点，从来自六个地缘政治区域的患者中采集了 1281 份 HBsAg 阳性样本，进行了一项全国性研究。使用商业 ELISA 检测试剂盒和实时 RT-PCR 试剂盒分别进行抗-HDV 抗体 (HDV-Ab) 筛查和 HDV-RNA 病毒载量定量。通过对病毒基因组所谓的 区域的扩增子进行 Sanger 测序，随后进行系统发育分析，进行 HDV 基因分型。在 1281 份 HBsAg 阳性样本中，有 61 份 (4.8%) 抗-HDV-Ab 阳性，其中 12 份 (19.7%) 抗-HDV-RNA 阳性。对其中的 9 份进行了基因分型：7 株“非洲”HDV-1、1 株“亚洲/欧洲”HDV-1 和 1 株 HDV-6。本研究表明，尼日利亚是一个 HDV 低流行率的国家，主要流行“非洲”基因型 1 株。
