Wu J C, Chiang T Y, Sheen I J
Department of Medicine, Veterans General Hospital, National Yang-Ming University School of Medicine, Taipei, Taiwan, Republic of China.
J Gen Virol. 1998 May;79 ( Pt 5):1105-13. doi: 10.1099/0022-1317-79-5-1105.
The hepatitis D virus (HDV) genotypes in 46 HDV-infected patients and 12 prostitutes were screened with Xhol restriction fragment length polymorphism (RFLP) analysis of reverse transcription PCR products of viral genomes and verified by phylogenetic analysis. The amplificates of three (6.5%) patients and two (17%) prostitutes showed a novel RFLP pattern different from those of the three known genotypes. Complete HDV genomic sequence identities between isolates with a novel RFLP and the HDV genotypes I, II and III were 72.3, 77.2 and 63.0%, respectively. Importantly, divergence was mostly seen in various regions related to replication or packaging. The novel isolates formed a monophyletic group (P < 0.05) and were most closely related to genotype II.
采用病毒基因组逆转录聚合酶链反应（RT-PCR）产物的Xhol限制性片段长度多态性（RFLP）分析，对46例丁型肝炎病毒（HDV）感染患者和12名妓女的HDV基因型进行筛查，并通过系统发育分析进行验证。3例（6.5%）患者和2例（17%）妓女的扩增产物显示出一种不同于三种已知基因型的新型RFLP模式。具有新型RFLP的分离株与HDV基因型I、II和III之间的完整HDV基因组序列同一性分别为72.3%、77.2%和63.0%。重要的是，差异主要出现在与复制或包装相关的各个区域。新型分离株形成一个单系群（P<0.05），与基因型II关系最为密切。
