Carnegie Institution of Washington, Department of Plant Biology, Stanford, California 94305.
Plant Physiol. 1982 Oct;70(4):1004-8. doi: 10.1104/pp.70.4.1004.
Illumination of intact Bryopsis corticulans chloroplasts under anaerobic conditions induced a decline of chlorophyll fluorescence and photoinhibition of Photosystems I and II. The time course of the light-induced decline of chlorophyll fluorescence and the decreases of activities of reactions sensitized by Photosystems I and II were compared. Photosystem I activity decreased in parallel with the disappearance of active P700. The time course of the destruction of the reaction center of Photosystem II was similar to that of photoinhibition of 2,6-dichlorophenolindophenol-Hill reaction.It appears that the initial events in photoinhibition are the destruction of the reaction centers of Photosystems I and II and that the reaction centers that are inhibited become quenchers of chlorophyll fluorescence.Effects of inhibitors of electron transfer and of an electron donor to Photosystem I showed that photoinhibition was related to Photosystem I activity.
在厌氧条件下对完整的石莼叶绿体进行光照会导致叶绿素荧光衰减和光系统 I 和 II 的光抑制。比较了光诱导的叶绿素荧光衰减和光系统 I 和 II 敏化反应活性降低的时间过程。光系统 I 活性随着活性 P700 的消失而平行下降。光系统 II 反应中心破坏的时间过程与 2,6-二氯苯酚靛酚-Hill 反应的光抑制相似。看来,光抑制的初始事件是光系统 I 和 II 的反应中心的破坏,而被抑制的反应中心成为叶绿素荧光的猝灭剂。电子传递抑制剂和光系统 I 的电子供体的作用表明,光抑制与光系统 I 的活性有关。