Doerr J A, Wyatt R D, Hamilton P B
Poult Sci. 1975 Jul;54(4):969-80. doi: 10.3382/ps.0540969.
This investigation was undertaken to standardize the determination of the one-stage prothrombin time for use with chickens. Homologous thromboplastin was essential and the most active thromboplastin was obtained from chickens four-weeks old or younger. Acetone-dried brain powder could be stored for at least 4 months at -15 degrees C. without loss of activity. Extraction of brain powder with 0.025 M CaCl2 at 42 degrees C. gave better thromboplastic activity than the standard extraction with physiological saline at room temperature. Thromboplastin solutions could be stored in ice water for only 6 hours without loss of activity. Citrate concentration had to be increased from the usual 0.10 M to 0.18 M to prevent premature clotting of plasma. Plasma donor age had no effect on the prothrombin times. Freezing and thawing as well as storage of plasma in the frozen state increased the prothrombin times. Using the best conditions, the mean prothrombin time for 1200 birds determined over a 6-month period was 9.4 sec. with an individual range of 7.18-11.4 sec. This represents a significantly lower prothrombin time with lower variability than that reported in the literature.
进行这项研究是为了规范鸡的一期凝血酶原时间测定方法。同源凝血活酶至关重要，最具活性的凝血活酶是从四周龄及以下的鸡中获得的。丙酮干燥脑粉在-15℃下可保存至少4个月而不失活。在42℃下用0.025M氯化钙提取脑粉比在室温下用生理盐水进行标准提取具有更好的凝血活性。凝血活酶溶液在冰水中只能保存6小时而不失活。柠檬酸盐浓度必须从通常的0.10M提高到0.18M，以防止血浆过早凝固。血浆供体的年龄对凝血酶原时间没有影响。冷冻和解冻以及血浆在冷冻状态下的储存会延长凝血酶原时间。在最佳条件下，6个月内测定的1200只鸡的平均凝血酶原时间为9.4秒，个体范围为7.18-11.4秒。这表明凝血酶原时间明显更低，变异性也比文献报道的要低。
