人类和实验动物淋巴细胞性脉络丛脑膜炎病毒爆发的实验室研究。

Laboratory studies of a lymphocytic choriomeningitis virus outbreak in man and laboratory animals.

作者信息

Bowen G S, Calisher C H, Winkler W G, Kraus A L, Fowler E H, Garman R H, Fraser D W, Hinman A R

出版信息

Am J Epidemiol. 1975 Sep;102(3):233-40. doi: 10.1093/oxfordjournals.aje.a112152.

DOI:10.1093/oxfordjournals.aje.a112152

Abstract

Investigation of an outbreak of prolonged febrile illness in medical center personnel at the University of Rochester School of Medicine and Dentistry revealed lymphocytic choriomeningitis (LCM) virus to be the causative agent. Syrian or golden hamsters (Mesocricetus auratus) were found to be the only animals involved in maintaining the virus and were the source of human infections. Isolations of LCM virus were made from autopsy specimens of 13 of 46 (28%) golden hamsters. Virus isolations were made from 22 of 28 (79%) frozen specimens of 11 tumor lines transplanted repeatedly in golden hamster cheek pouches. No virus isolations were made from 86 autopsied laboratory mice, laboratory rats, Chinese hamsters (Cricetulus griseus), or laboratory rabbits or from 10 tumor cell lines transplanted in laboratory mice. Complement-fixation testing of 301 animal sera from the vivarium also revealed involvement primarily of golden hamsters. The probable source of virus introduction into the Rochester facilities was found to be two LCM-contaminated tumor lines sent from a biological supplier to Rochester in 1969.

摘要

对罗切斯特大学医学与牙科学院医学中心工作人员中爆发的持续性发热疾病的调查显示，淋巴细胞性脉络丛脑膜炎（LCM）病毒是病原体。叙利亚仓鼠或金黄仓鼠（Mesocricetus auratus）被发现是唯一参与维持该病毒的动物，也是人类感染的来源。从46只金黄仓鼠中的13只（28%）的尸检标本中分离出了LCM病毒。从在金黄仓鼠颊囊中反复移植的11个肿瘤系的28个（79%）冷冻标本中分离出了病毒。在86只经尸检的实验室小鼠、大鼠、中国仓鼠（Cricetulus griseus）或实验兔中未分离出病毒，在移植到实验室小鼠中的10个肿瘤细胞系中也未分离出病毒。对饲养室的301份动物血清进行补体结合试验也显示主要涉及金黄仓鼠。发现病毒引入罗切斯特设施的可能来源是1969年一家生物供应商送到罗切斯特的两个受LCM污染的肿瘤系。