Betelak K F, Margiotti E A, Wohlford M E, Suzuki D A
Department of Ophthalmology, Indiana University, Indianapolis, IN 46202-5143, USA.
J Neurosci Methods. 2001 Nov 15;112(1):9-20. doi: 10.1016/s0165-0270(01)00442-3.
Investigators that require the chronic implantation of hardware on the non-human primate skull are often challenged with the possible failure of head implants. To improve the success rate of our head implants, titanium anchor screws, referred to as titanium endosseous implants, were implanted in the skulls of six macaques. Techniques adapted from the processing of dentures were utilized to pre-fabricate an acrylic "skull cap", which provided the mechanical support structure for our study-specific hardware. A two-stage procedure adapted from dentistry (Brånemark et al., 1977) was employed for the placement of titanium endosseous implants in the skull. This two-stage technique incorporates the principles of osseointegration and a healing period. Of the six skull-anchored implants prepared implementing the methods described in this paper, we have not experienced any failures. Additionally, all of the titanium endosseous implants examined post mortem were functionally successful (n=30). Histology results confirmed that there was healthy bone in direct contact with the titanium endosseous implants. The dense cortical bone of the macaque skull is ideal for the implantation of titanium endosseous implants. Titanium endosseous implants have provided secure, functional anchor points for the attachment of hardware to the macaque skull and have resulted in healthy, stable head implants that can remain on the skull for extended periods of time.
需要在非人灵长类动物头骨上长期植入硬件的研究人员常常面临头部植入物可能失败的挑战。为了提高我们头部植入物的成功率，我们将钛锚钉（称为钛骨内植入物）植入了六只猕猴的头骨中。采用了假牙加工过程中的技术来预制一个丙烯酸“头盖骨”，它为我们特定研究的硬件提供了机械支撑结构。采用了一种源自牙科的两阶段手术方法（Brånemark等人，1977年）将钛骨内植入物放置在头骨中。这种两阶段技术融合了骨整合原理和愈合期。在按照本文所述方法制备的六个头骨锚定植入物中，我们没有遇到任何失败情况。此外，所有死后检查的钛骨内植入物在功能上都是成功的（n = 30）。组织学结果证实，有健康的骨头与钛骨内植入物直接接触。猕猴头骨的致密皮质骨非常适合植入钛骨内植入物。钛骨内植入物为将硬件附着到猕猴头骨上提供了安全、功能性的锚定点，并产生了健康、稳定的头部植入物，这些植入物可以在头骨上保留很长时间。
