Raushenbakh I Iu, Gruntenko N E, Chentsova N A, Hirashima A, Sukhanova M Zh, Andreenkova E V, Glazko G V
Institute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk, 630090 Russia.
Genetika. 2001 Sep;37(9):1243-50.
The effect of heat stress (38 degrees C) on the content of octopamine (OA) and 20-hydroxyecdysone (20HE) was studied under normal and stressful conditions in adult flies of Drosophila virilis lines contrasting in the level of the juvenile hormone (JH). The wild-type flies (line 101) exhibited a pronounced sex dimorphism for the content of both OA and 20HE, which was substantially lower in this line than in flies of the mutant line 147. The level of both hormones increased in flies of line 101 exposed to heat stress, whereas it remained unchanged in flies of line 147 under the same conditions. The effect of heat stress on the level of JH metabolism and fertility was also studied in D. melanogaster wild-type lines and lines carrying mutations in genes responsible for OA and DA syntheses. In octopamineless females of the T beta hnM18 line and in females of the Ste line characterized by a doubled content of DA, JH degradation differed from normal: it was increased in both young and mature T beta hnM18 females, while decreased in young and increased in mature Ste flies. Fertility was substantially lower in the Ste than in the wild-type line. Flies of all of the D. melanogaster lines produced a stress response; however, in mutant lines, both fertility and stress reactivity of the systems controlling JH metabolism differed significantly from that of the wild-type lines. The role of JH, 20HE, OA, and DA interaction in regulation of Drosophila reproduction under stressful conditions is discussed.
在正常和应激条件下，研究了热应激（38摄氏度）对不同保幼激素（JH）水平的粗壮果蝇成年果蝇体内章鱼胺（OA）和20-羟基蜕皮酮（20HE）含量的影响。野生型果蝇（品系101）在OA和20HE含量上表现出明显的性别二态性，该品系中的这两种物质含量显著低于突变品系147中的果蝇。暴露于热应激下的101品系果蝇体内这两种激素的水平均升高，而在相同条件下147品系果蝇体内的激素水平保持不变。还在黑腹果蝇野生型品系以及携带负责OA和多巴胺（DA）合成基因发生突变的品系中，研究了热应激对JH代谢水平和繁殖力的影响。在TβhnM18品系的无章鱼胺雌性果蝇以及DA含量加倍的Ste品系雌性果蝇中，JH降解情况与正常情况不同：在年轻和成熟的TβhnM18雌性果蝇中JH降解均增加，而在年轻的Ste果蝇中JH降解减少，在成熟的Ste果蝇中JH降解增加。Ste品系果蝇的繁殖力显著低于野生型品系。所有黑腹果蝇品系的果蝇都会产生应激反应；然而，在突变品系中，控制JH代谢的系统的繁殖力和应激反应性均与野生型品系有显著差异。文中讨论了JH、20HE、OA和DA相互作用在应激条件下对果蝇繁殖调节中的作用。
