在意大利北部分离出的牛病毒性腹泻病毒2型毒株的基因型特征
Genotypic characteristics of bovine viral diarrhea virus 2 strains isolated in northern Italy.
作者信息
Giangaspero M, Harasawa R, Zecconi A, Luzzago C
机构信息
Institute of Special Pathology and Veterinary Medical Clinic, The University of Milan, Italy.
出版信息
J Vet Med Sci. 2001 Sep;63(9):1045-9. doi: 10.1292/jvms.63.1045.
Two strains of Bovine viral diarrhea virus 2 (BVDV-2) were isolated from calves in northern Italy. Variations in the 5'-untranslated region (UTR) of the genome were studied by primary structure alignment and neighbor-joining method based phylogenetic tree analyses and by palindromic nucleotide substitutions at the three variable loci in the 5'-UTR. Genetic analysis indicated their appurtenance to genovar BVDV-2a. Nucleotide sequence at the 5'-UTR of strain BS-95-II, one of the Italian isolates from healthy calves, showed 98% homology to that of the Japanese isolate OY89, a cytopathic strain derived from cattle with mucosal disease.
从意大利北部的犊牛中分离出两株牛病毒性腹泻病毒2型（BVDV - 2）。通过一级结构比对、基于邻接法的系统发育树分析以及5'-非翻译区（UTR）三个可变位点的回文核苷酸替换，研究了基因组5'-UTR的变异情况。遗传分析表明它们属于基因变种BVDV - 2a。来自健康犊牛的意大利分离株之一BS - 95 - II的5'-UTR核苷酸序列与日本分离株OY89（一种源自患有黏膜病牛的细胞病变株）的序列显示出98%的同源性。
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