韩国分离的牛病毒性腹泻病毒5'-非翻译区的比较分析

Comparative analysis on the 5'-untranslated region of bovine viral diarrhea virus isolated in Korea.

作者信息

Park J S, Moon H J, Lee B C, Hwang W S, Yoo H S, Kim D Y, Park B K

机构信息

Department of Veterinary Medicine Virology Lab, The Xenotransplantation Research Center, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, 151-742, Seoul, Republic of Korea.

出版信息

Res Vet Sci. 2004 Apr;76(2):157-63. doi: 10.1016/j.rvsc.2003.10.003.

DOI:10.1016/j.rvsc.2003.10.003

PMID:14672860

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7127663/

Abstract

Samples of 249 bovine abortuses, one intestine, and four diarrheal stools from 254 cows were collected, and bovine viral diarrhea virus (BVDV) was detected by reverse transcription-polymerase chain reaction (RT-PCR). Subsequently, virus isolation was preformed with PCR-positive samples, and then PCR product of 5(')-untranslated region (UTR) of BVDV isolate was sequenced and analyzed. Among the samples collected, 20 (from 17 bovine abortuses, one intestine, and two diarrheal stools) were positive for BVDV RT-PCR; four BVDVs (from two bovine abortuses, one intestine, and one diarrheal stool) were isolated. When the four isolates were biotyped in cell culture, one BVDV isolate from a bovine abortus was cytopathic and the others were non-cytopathic. In addition, three isolates were genotyped as BVDV-1 and one isolate from a diarrheal stool as BVDV-2. In phylogenetic analysis, it suggested that the BVDV-2 isolate in Korea is closer to the North American strains than Asian strains. This is the first report on the identification and isolation of BVDV-2 in Korea.

摘要

收集了249份牛流产样本、1份肠道样本以及来自254头牛的4份腹泻粪便样本，通过逆转录-聚合酶链反应（RT-PCR）检测牛病毒性腹泻病毒（BVDV）。随后，对PCR阳性样本进行病毒分离，然后对BVDV分离株5(')-非翻译区（UTR）的PCR产物进行测序和分析。在所收集的样本中，20份（来自17份牛流产样本、1份肠道样本和2份腹泻粪便样本）BVDV RT-PCR呈阳性；分离出4株BVDV（来自2份牛流产样本、1份肠道样本和1份腹泻粪便样本）。当在细胞培养中对这4株分离株进行生物分型时，1株来自牛流产样本的BVDV分离株具有细胞病变效应，其他分离株无细胞病变效应。此外，3株分离株被基因分型为BVDV-1，1株来自腹泻粪便的分离株为BVDV-2。在系统发育分析中，结果表明韩国的BVDV-2分离株与北美毒株的亲缘关系比与亚洲毒株更近。这是韩国关于BVDV-2鉴定和分离的首次报道。