Heavner S B, Hardy S M, White D R, Prazma J, Pillsbury H C
Department of Surgery, University of North Carolina School of Medicine, University of North Carolina Hospitals, Chapel Hill, USA.
Ann Otol Rhinol Laryngol. 2001 Oct;110(10):928-34. doi: 10.1177/000348940111001007.
Gastroesophageal reflux is a common problem in the newborn and preschool periods. Recent research suggests that it may be related to eustachian tube dysfunction and otitis media with effusion. The purpose of this experiment was to investigate the relationship between simulated gastroesophageal reflux and eustachian tube dysfunction. Rat middle ears were repeatedly exposed (transtympanically) to pepsin in hydrochloric acid or to phosphate-buffered saline solution. Their eustachian tube function was evaluated by assessing passive opening and passive closing pressures, and active clearance of positive and negative pressure. The passive pressure function tests showed variable results. The rats exposed to pepsin in hydrochloric acid had an impaired ability to clear positive and negative pressure from the middle ear as compared to the rats exposed only to phosphate-buffered saline solution. The results demonstrate that multiple middle ear exposures to pepsin in hydrochloric acid leads to eustachian tube dysfunction in rats.
胃食管反流是新生儿期和学龄前期的常见问题。最近的研究表明，它可能与咽鼓管功能障碍及分泌性中耳炎有关。本实验的目的是研究模拟胃食管反流与咽鼓管功能障碍之间的关系。将大鼠中耳经鼓膜反复暴露于盐酸胃蛋白酶或磷酸盐缓冲盐溶液中。通过评估被动开放压、被动关闭压以及正负压的主动清除情况来评价其咽鼓管功能。被动压力功能测试结果各异。与仅暴露于磷酸盐缓冲盐溶液的大鼠相比，暴露于盐酸胃蛋白酶的大鼠清除中耳正负压的能力受损。结果表明，大鼠中耳多次暴露于盐酸胃蛋白酶会导致咽鼓管功能障碍。
