Department of ENT, Amrita Institute of Medical Sciences and Research Centre, (AIMS), Ponekkara P.O., Kochi, Kerala 682041, India.
Indian J Pediatr. 2012 Oct;79(10):1328-32. doi: 10.1007/s12098-011-0671-y. Epub 2012 Jan 7.
To establish whether Gastroesophageal reflux disease (GERD) plays a significant role in the etiopathogenesis of otitis media with effusion (OME) patients and to find if there is any association between GERD symptoms and OME. Also, to detect presence of pepsinogen in the middle ear aspirate and to demonstrate pepsinogen band by electrophoresis.
This is a prospective study conducted in a tertiary care hospital. Middle ear effusion samples were collected from 32 children undergoing surgery for OME.The samples were measured for pepsinogen level as well as total protein content. Samples were subjected to electrophoresis study by SDS PAGE (Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis).
A total of 21 patients out of 32 were found to have higher pepsinogen level in their middle ear effusion samples than the cut off value of 90 mg/ml.The lack of symptoms associated with GERD among the children in this study corroborates with other similar studies in the world literature. It was also statistically shown that the association between GERD score and pepsinogen level was insignificant (P = 0.512).
OME appears to be multifactorial disease.GERD may be one of the contributing factors in the etiopathogenisis of OME.
确定胃食管反流病（GERD）是否在分泌性中耳炎（OME）患者的病因发病机制中起重要作用，并发现 GERD 症状与 OME 是否存在任何关联。此外，还检测中耳吸出物中胃蛋白酶原的存在，并通过电泳检测胃蛋白酶原条带。
这是一项在三级护理医院进行的前瞻性研究。收集 32 例因 OME 接受手术的儿童的中耳积液样本。测量样本中的胃蛋白酶原水平和总蛋白含量。通过 SDS PAGE（十二烷基硫酸钠聚丙烯酰胺凝胶电泳）对样本进行电泳研究。
在 32 名患者中，共有 21 名患者的中耳积液样本中的胃蛋白酶原水平高于 90mg/ml 的临界值。在这项研究中，儿童中与 GERD 相关的症状缺失与世界文献中的其他类似研究相符。统计学还表明，GERD 评分与胃蛋白酶原水平之间的关联无统计学意义（P=0.512）。
OME 似乎是一种多因素疾病。GERD 可能是 OME 病因发病机制的一个因素。
