Lane M, Hooper K, Gardner D K
Colorado Center for Reproductive Medicine, Research and Development, Englewood, Colorado, USA.
J Assist Reprod Genet. 2001 Sep;18(9):519-25. doi: 10.1023/a:1016657228171.
To examine the effect of essential amino acids concentrations on mouse embryo development.
Mouse embryos were cultured in medium with different concentrations of essential amino acids and development to the blastocyst stage and viability assessed. Ammonium production resulting from medium breakdown and amino acid metabolism by embryos were also assessed.
Reducing the essential amino acid concentration significantly increased blastocyst development and cell numbers. Lowering the essential amino acid concentration decreased ammonium production in the medium.
Culture media for the development of preimplantation embryos should have a reduced essential amino acid concentration to facilitate embryo development.
研究必需氨基酸浓度对小鼠胚胎发育的影响。
将小鼠胚胎培养于含有不同浓度必需氨基酸的培养基中，评估其发育至囊胚阶段的情况及活力。同时评估胚胎对培养基分解及氨基酸代谢产生的铵的生成情况。
降低必需氨基酸浓度显著提高了囊胚发育及细胞数量。降低必需氨基酸浓度减少了培养基中铵的生成。
用于植入前胚胎发育的培养基应降低必需氨基酸浓度以促进胚胎发育。
