Patt L M, Van Nest G A, Grimes W J
Cancer Res. 1975 Feb;35(2):438-41.
The ability of suspensions of BALB/c cells to catalyze the incorporation of nucleotide sugars into complex polysaccharides has been compared. These cells have previously been characterized for concanavalin A-induced agglutinability, tumorigenicity, and malignancy. All of the cell lines tested catalyze transfer of the sugar moieties of cytosine 5-monophosphate N-acetylneuraminic acid, uridine 5-diphosphate galactose, uridine 5-diphosphate N-acetylgalactosamine, uridine 5-diphosphate N-acetylglucosamine, uridine 5-diphosphate glucose, and guanidine 5-diphosphate monnose into glycoproteins and glycolipids. While some transformed lines exhibit alterations in transferase levels, others cannot be distinguished from normal cells. Normal cells, transformed cells that cause tumors that regress, and transformed cells that cause tumors that kill an immunologically competent host show growth-dependent changes in transferase activities. Determining the ability to catalyze carbohydrate transfer is insufficient for predicting the tumorigenic and malignant properties of a cell line.
已对BALB/c细胞悬液催化核苷酸糖掺入复合多糖的能力进行了比较。这些细胞此前已根据伴刀豆球蛋白A诱导的凝集性、致瘤性和恶性程度进行了表征。所有测试的细胞系都能催化5-磷酸胞苷N-乙酰神经氨酸、5-二磷酸尿苷半乳糖、5-二磷酸尿苷N-乙酰半乳糖胺、5-二磷酸尿苷N-乙酰葡糖胺、5-二磷酸尿苷葡萄糖和5-二磷酸胍甘露糖的糖基转移至糖蛋白和糖脂中。虽然一些转化细胞系的转移酶水平有变化，但其他细胞系与正常细胞并无区别。正常细胞、能使肿瘤消退的转化细胞以及能使肿瘤杀死免疫活性宿主的转化细胞，其转移酶活性呈现出与生长相关的变化。确定催化碳水化合物转移的能力不足以预测细胞系的致瘤性和恶性特性。
