Ohyama M, Otake T, Morinaga K
Osaka Prefectural Institute of Public Health, 1-3-69 Nakamichi, Higashinari-ku, Osaka 537-0025, Japan.
Environ Health Perspect. 2001 Oct;109(10):1033-8. doi: 10.1289/ehp.011091033.
Fiber size is an important factor in the tumorigenicity of various mineral fibers and asbestos fibers in animal experiments. We examined the time course of the ability to induce lucigenin-dependent chemiluminescence (CL) from human monocyte-derived macrophages exposed to Japan Fibrous Material standard reference samples (glass wool, rock wool, micro glass fiber, two types of refractory ceramic fiber, refractory mullite fiber, potassium titanium whisker, silicon carbide whisker, titanium oxide whisker, and wollastonite). We determined how fiber length or width might modify the response of cells. We found that the patterns of time-dependent increase of CL (sigmoid type) were similar for each sample except wollastonite. We observed a strong correlation between geometric-mean length and ability to induce CL in seven samples > 6 microm in length over the time course (largest r(2) = 0.9760). Although we also observed a close positive correlation between geometric-mean width and the ability to induce CL in eight samples < 1.8 microm in width at 15 min (r(2) = 0.8760), a sample of 2.4 microm in width had a low ability to induce CL. Moreover, the relationship between width and the rate of increase in ability to induce CL had a negative correlation at 30-60 min (largest r(2) = 0.7473). Our findings suggest that the release of superoxide from macrophages occurs nonspecifically for various types of mineral fibers depending on fiber length.
在动物实验中，纤维尺寸是各种矿物纤维和石棉纤维致瘤性的一个重要因素。我们研究了暴露于日本纤维材料标准参考样品（玻璃棉、岩棉、微玻璃纤维、两种耐火陶瓷纤维、耐火莫来石纤维、钛酸钾晶须、碳化硅晶须、氧化钛晶须和硅灰石）的人单核细胞衍生巨噬细胞诱导光泽精依赖性化学发光（CL）能力的时间进程。我们确定了纤维长度或宽度如何改变细胞的反应。我们发现，除硅灰石外，每个样品CL随时间增加的模式（S型）相似。在整个时间进程中，我们观察到七种长度大于6微米的样品的几何平均长度与诱导CL的能力之间存在很强的相关性（最大r² = 0.9760）。尽管我们还观察到在15分钟时，八种宽度小于1.8微米的样品的几何平均宽度与诱导CL的能力之间存在密切的正相关（r² = 0.8760），但一个宽度为2.4微米的样品诱导CL的能力较低。此外，在30 - 60分钟时，宽度与诱导CL能力的增加速率之间的关系呈负相关（最大r² = 0.7473）。我们的研究结果表明，巨噬细胞中超氧化物的释放对于各种类型的矿物纤维是非特异性的，这取决于纤维长度。
