Hishikawa N, Hashizume Y, Yoshida M, Sobue G
Department of Neurology, Nagoya University School of Medicine, Showa-ku, Nagoya, Japan.
Neuropathol Appl Neurobiol. 2001 Oct;27(5):362-72. doi: 10.1046/j.1365-2990.2001.00345.x.
Argyrophilic glial inclusions, which are immunohistochemically positive for alpha-synuclein but negative for tau protein, were examined in the brain of Parkinson's disease (PD) patients. Autopsied brains of 10 individuals who died from PD, of two incidental Lewy body disease cases and of five age-matched individuals whose deaths were caused by non-neurological diseases were studied, histopathologically, by Gallyas-Braak staining and, immunohistochemically, with anti-alpha-synuclein antibody, anti-ubiquitin, and anti-tyrosine hydroxylase. All postmortem PD brains showed a significant number of argyrophilic glial inclusions, but no glial inclusions were found in control brains. The inclusions were found not only in the regions showing neuronal loss and gliosis, such as the substantia nigra, locus ceruleus and dorsal vagal nucleus, but also in regions without neuronal loss and gliosis, such as the cerebral cortex, cerebral white matter, striatum, globus pallidus, thalamus, cerebellum and spinal cord. The distribution and density of glial inclusions in PD brains varied from case to case but, in the cerebral cortex, the number of glial inclusions were fairly well correlated with the number of Lewy bodies. The distribution pattern of glial inclusions also showed a striking resemblance to that of catecholaminergic neurones and fibres. The abnormal accumulation of alpha-synuclein in glial cells was more widespread than neurone loss, and appears to be an important pathological feature of PD.
对帕金森病（PD）患者大脑中嗜银性胶质包涵体进行了检查，这些包涵体α-突触核蛋白免疫组化呈阳性，但tau蛋白免疫组化呈阴性。对10例死于PD的个体、2例偶发性路易体病病例以及5例死于非神经系统疾病的年龄匹配个体的尸检大脑进行了研究，采用Gallyas-Braak染色进行组织病理学检查，并用抗α-突触核蛋白抗体、抗泛素和抗酪氨酸羟化酶进行免疫组化检查。所有PD死后大脑均显示大量嗜银性胶质包涵体，但对照大脑中未发现胶质包涵体。这些包涵体不仅在显示神经元丢失和胶质增生的区域，如黑质、蓝斑和迷走神经背核中发现，而且在没有神经元丢失和胶质增生的区域，如大脑皮质、脑白质、纹状体、苍白球、丘脑、小脑和脊髓中也发现。PD大脑中胶质包涵体的分布和密度因病例而异，但在大脑皮质中，胶质包涵体的数量与路易体的数量相当相关。胶质包涵体的分布模式也与儿茶酚胺能神经元和纤维的分布模式惊人地相似。α-突触核蛋白在胶质细胞中的异常积累比神经元丢失更为广泛，似乎是PD的一个重要病理特征。
