恶性疟原虫红细胞结合抗原（EBA - 175）：通过巢式聚合酶链反应改进基因型测定
Erythrocyte binding antigen (EBA-175) of Plasmodium falciparum: improved genotype determination by nested polymerase chain reaction.
作者信息
Touré F S, Mavoungou E, Ndong J M, Tshipamba P, Deloron P
机构信息
Centre International de Recherches Médicales de Franceville, Franceville, Gabon.
出版信息
Trop Med Int Health. 2001 Oct;6(10):767-9. doi: 10.1046/j.1365-3156.2001.00789.x.
Abstract
We have designed primers to the conserved region of the erythrocyte binding antigen (EBA)-175 gene which amplify specifically the two alleles by polymerase chain reaction (PCR) and by nested PCR. This approach provides a specific, sensitive and rapid method for genotype determination in a large number of Plasmodium falciparum field isolates.
摘要
我们已针对红细胞结合抗原（EBA）-175基因的保守区域设计了引物，这些引物可通过聚合酶链反应（PCR）和巢式PCR特异性扩增两个等位基因。该方法为大量恶性疟原虫野外分离株的基因型测定提供了一种特异、灵敏且快速的方法。
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