焦磷酸测序法作为一种基于内化素B基因单核苷酸多态性对单核细胞增生李斯特菌菌株进行分组的方法。
Pyrosequencing as a method for grouping of Listeria monocytogenes strains on the basis of single-nucleotide polymorphisms in the inlB gene.
作者信息
Unnerstad H, Ericsson H, Alderborn A, Tham W, Danielsson-Tham M L, Mattsson J G
机构信息
Department of Food Hygiene, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden.
出版信息
Appl Environ Microbiol. 2001 Nov;67(11):5339-42. doi: 10.1128/AEM.67.11.5339-5342.2001.
Abstract
By using pyrosequencing (i.e., sequencing by synthesis) 106 strains of different serovars of Listeria monocytogenes were rapidly grouped into four categories based on nucleotide variations at positions 1575 and 1578 of the inlB gene. Strains of serovars 1/2a and 1/2c constituted one group, and strains of serovars 1/2b and 3b constituted another group, whereas serovar 4b strains were separated into two groups.
摘要
通过焦磷酸测序法（即边合成边测序），根据内化素B（inlB）基因第1575位和第1578位的核苷酸变异，将106株不同血清型的单核细胞增生李斯特菌迅速分为四类。血清型1/2a和1/2c的菌株构成一组，血清型1/2b和3b的菌株构成另一组，而血清型4b的菌株则分为两组。
相似文献
1
Pyrosequencing as a method for grouping of Listeria monocytogenes strains on the basis of single-nucleotide polymorphisms in the inlB gene.焦磷酸测序法作为一种基于内化素B基因单核苷酸多态性对单核细胞增生李斯特菌菌株进行分组的方法。
Appl Environ Microbiol. 2001 Nov;67(11):5339-42. doi: 10.1128/AEM.67.11.5339-5342.2001.
2
3
Molecular grouping of Listeria monocytogenes based on the sequence of the inIB gene.基于inIB基因序列的单核细胞增生李斯特菌分子分型
J Med Microbiol. 2000 Jan;49(1):73-80. doi: 10.1099/0022-1317-49-1-73.
4
Comparative characterization of Listeria monocytogenes isolated from Portuguese farmhouse ewe's cheese and from humans.从葡萄牙农家母羊奶酪和人类中分离出的单核细胞增生李斯特菌的比较特征分析。
Int J Food Microbiol. 2006 Feb 1;106(2):111-21. doi: 10.1016/j.ijfoodmicro.2005.05.017. Epub 2005 Oct 10.
5
6
Low genetic diversity and epidemiological significance of Listeria monocytogenes isolated from wild animals in the far east of Russia.俄罗斯远东地区野生动物分离出的单核细胞增生李斯特菌的低遗传多样性及流行病学意义
Infect Genet Evol. 2007 Dec;7(6):736-42. doi: 10.1016/j.meegid.2007.07.006. Epub 2007 Jul 20.
7
Detection of Listeria monocytogenes by polymerase chain reaction oriented to inlB gene.基于inlB基因的聚合酶链反应检测单核细胞增生李斯特菌
New Microbiol. 2001 Oct;24(4):333-9.
8
Molecular characteristics and virulence potential of Listeria monocytogenes isolates from Chinese food systems.来自中国食品体系的单核细胞增生李斯特菌分离株的分子特征及毒力潜力
Food Microbiol. 2009 Feb;26(1):103-11. doi: 10.1016/j.fm.2008.08.003. Epub 2008 Oct 18.
9
Combined sigB allelic typing and multiplex PCR provide improved discriminatory power and reliability for Listeria monocytogenes molecular serotyping.联合sigB等位基因分型和多重PCR可提高单核细胞增生李斯特菌分子血清分型的鉴别能力和可靠性。
J Microbiol Methods. 2007 Jan;68(1):52-9. doi: 10.1016/j.mimet.2006.06.005. Epub 2006 Aug 2.
10
Genetic diversity of Listeria monocytogenes recovered from infected persons and pork, seafood and dairy products on retail sale in France during 2000 and 2001.2000年至2001年期间从法国零售的受感染人员以及猪肉、海鲜和乳制品中分离出的单核细胞增生李斯特菌的遗传多样性。
Int J Food Microbiol. 2007 Mar 10;114(2):187-94. doi: 10.1016/j.ijfoodmicro.2006.09.011. Epub 2006 Dec 26.
引用本文的文献
1
Rapid Detection and Identification of Yersinia pestis from Food Using Immunomagnetic Separation and Pyrosequencing.利用免疫磁珠分离和焦磷酸测序技术从食品中快速检测和鉴定鼠疫耶尔森菌
J Pathog. 2012;2012:781652. doi: 10.1155/2012/781652. Epub 2012 Oct 3.
2
Gene Scanning of an Internalin B Gene Fragment Using High-Resolution Melting Curve Analysis as a Tool for Rapid Typing of Listeria monocytogenes.应用高分辨率熔解曲线分析技术对 Internalin B 基因片段进行基因扫描，快速分型李斯特菌。
J Mol Diagn. 2011 Jan;13(1):57-63. doi: 10.1016/j.jmoldx.2010.11.002. Epub 2010 Dec 23.
3
Pyrosequencing using the single-nucleotide polymorphism protocol for rapid determination of TEM- and SHV-type extended-spectrum beta-lactamases in clinical isolates and identification of the novel beta-lactamase genes blaSHV-48, blaSHV-105, and blaTEM-155.使用单核苷酸多态性协议进行焦磷酸测序，以快速测定临床分离株中TEM型和SHV型超广谱β-内酰胺酶，并鉴定新型β-内酰胺酶基因blaSHV-48、blaSHV-105和blaTEM-155。
Antimicrob Agents Chemother. 2009 Mar;53(3):977-86. doi: 10.1128/AAC.01155-08. Epub 2008 Dec 15.
4
Pyrosequencing: sequence typing at the speed of light.焦磷酸测序法：以光速进行序列分型。
Mol Biotechnol. 2004 Oct;28(2):129-37. doi: 10.1385/mb:28:2:129.
5
A novel method for preparing single-stranded DNA for pyrosequencing.一种用于焦磷酸测序的单链DNA制备新方法。
Mol Biotechnol. 2003 Jun;24(2):221-4. doi: 10.1385/mb:24:2:221.
本文引用的文献
1
Listeria monocytogenes in faeces from clinically healthy dairy cows in Sweden.瑞典临床健康奶牛粪便中的单核细胞增生李斯特菌。
Acta Vet Scand. 2000;41(2):167-71. doi: 10.1186/BF03549648.
2
Determination of single-nucleotide polymorphisms by real-time pyrophosphate DNA sequencing.通过实时焦磷酸DNA测序法测定单核苷酸多态性
Genome Res. 2000 Aug;10(8):1249-58. doi: 10.1101/gr.10.8.1249.
3
Molecular grouping of Listeria monocytogenes based on the sequence of the inIB gene.基于inIB基因序列的单核细胞增生李斯特菌分子分型
J Med Microbiol. 2000 Jan;49(1):73-80. doi: 10.1099/0022-1317-49-1-73.
4
Surface protein p104 is involved in adhesion of Listeria monocytogenes to human intestinal cell line, Caco-2.表面蛋白p104参与单核细胞增生李斯特菌与人肠道细胞系Caco-2的黏附。
J Med Microbiol. 1999 Feb;48(2):117-124. doi: 10.1099/00222615-48-2-117.
5
A DNA polymorphism discovery resource for research on human genetic variation.一个用于人类遗传变异研究的DNA多态性发现资源。
Genome Res. 1998 Dec;8(12):1229-31. doi: 10.1101/gr.8.12.1229.
6
A sequencing method based on real-time pyrophosphate.一种基于实时焦磷酸的测序方法。
Science. 1998 Jul 17;281(5375):363, 365. doi: 10.1126/science.281.5375.363.
7
Molecular characterization of an autolytic amidase of Listeria monocytogenes EGD.单核细胞增生李斯特菌EGD自溶酰胺酶的分子特征分析
Microbiology (Reading). 1998 May;144 ( Pt 5):1359-1367. doi: 10.1099/00221287-144-5-1359.
8
Internalin B promotes the replication of Listeria monocytogenes in mouse hepatocytes.内化素B促进单核细胞增生李斯特菌在小鼠肝细胞中的复制。
Infect Immun. 1997 Dec;65(12):5137-41. doi: 10.1128/iai.65.12.5137-5141.1997.
9
InlB: an invasion protein of Listeria monocytogenes with a novel type of surface association.内化素B：一种具有新型表面结合方式的单核细胞增生李斯特菌入侵蛋白。
Mol Microbiol. 1997 Jul;25(2):285-94. doi: 10.1046/j.1365-2958.1997.4621825.x.
10
WHO study on subtyping Listeria monocytogenes: results of phage-typing.世界卫生组织关于单核细胞增生李斯特菌分型的研究：噬菌体分型结果
Int J Food Microbiol. 1996 Oct;32(3):289-99. doi: 10.1016/s0168-1605(96)01143-9.
Zotero 插件