Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, Minnesota, USA.
University of Minnesota Informatics Institute, University of Minnesota, Minneapolis, Minnesota, USA.
mBio. 2019 Jan 29;10(1):e01605-18. doi: 10.1128/mBio.01605-18.
causes porcine proliferative enteropathy. This is an enteric disease characterized by thickening of the wall of the ileum that leads to decreased growth of animals and diarrhea. In this study, we investigated the host response to infection by performing transcriptomic and pathway analysis of intestinal tissue samples from groups of infected and noninfected animals at 14, 21, and 28 days postchallenge. At the peak of infection, when animals developed the most severe lesions, infected animals had higher levels of several gene transcripts involved in cellular proliferation and inflammation, including matrix metalloproteinase-7 (), transglutaminase-2 (), and oncostatin M (). Histomorphology also revealed general features of intestinal inflammation. This study identified important pathways associated with the host response in developing and resolving lesions due to infection. is among the most important enteric pathogens of swine, and it can also infect other mammalian species. Much is still unknown regarding its pathogenesis and the host response, especially at the site of infection. In this study, we uncovered several novel genes and pathways associated with infection. Differentially expressed transcripts, in addition to histological changes in infected tissue, revealed striking similarities between infection and cellular proliferation mechanisms described in some cancers and inflammatory diseases of the gastrointestinal tract. This research sheds important light into the pathogenesis of and the host response associated with the lesions caused by infection.
导致猪增生性肠病。这是一种以回肠壁增厚为特征的肠病，导致动物生长减少和腹泻。在这项研究中，我们通过对感染和未感染动物在感染后 14、21 和 28 天的肠组织样本进行转录组和途径分析，研究了宿主对感染的反应。在感染高峰期，当动物出现最严重的病变时，感染动物的几种参与细胞增殖和炎症的基因转录本水平较高，包括基质金属蛋白酶-7（）、转谷氨酰胺酶-2（）和肿瘤坏死因子-α（）。组织形态学也显示出肠道炎症的一般特征。本研究确定了与 感染引起的病变发展和消退相关的重要途径。是猪最重要的肠病原体之一，也可以感染其他哺乳动物。关于其发病机制和宿主反应，尤其是在感染部位，仍有许多未知之处。在这项研究中，我们发现了与感染相关的几个新基因和途径。差异表达的转录本，加上感染组织的组织学变化，揭示了 感染与一些癌症和胃肠道炎症性疾病中描述的细胞增殖机制之间的惊人相似性。这项研究为 感染的发病机制和与感染引起的病变相关的宿主反应提供了重要的启示。
