Patriotis P C, Querec T D, Gruver B N, Brown T R, Patriotis C
Division of Medical Science, Fox Chase Cancer Center, Philadelphia, PA 19111, USA.
Biotechniques. 2001 Oct;31(4):862, 864, 866-8, 870, 872. doi: 10.2144/01314mt04.
Determining the dynamics in the global regulation of gene expression holds the promise of bringing a better understanding of the processes that govern physiological cell growth regulation and its disruption during the development of disease. The advent for cDNA arrays has created the possibility for the parallel analysis of expression of thousands of genes in a given cell population, simultaneously. The level of expression of a given set of genes within the studied tissue corresponds to the intensity of a labeled cDNA probe synthesized from the studied tissue RNA and bound specifically to the cDNAs of the genes spotted on the array. The accurate extraction of gene expression intensity values is essential for further data analysis and the interpretation of the obtained results. Here, we describe a new array image-processing software developed in Microsoft Visual Basic, the ArrayExplorer, which provides a user-friendly, multiple-window interface and a number of automatic and manual features that facilitate a reliable, robust, and accurate extraction of gene intensity values from filter-array images.
确定基因表达全局调控中的动态变化，有望让人更好地理解那些控制生理细胞生长调控及其在疾病发展过程中被破坏的过程。cDNA阵列的出现使得同时对给定细胞群体中数千个基因的表达进行平行分析成为可能。在所研究组织内给定一组基因的表达水平，对应于从所研究组织RNA合成并特异性结合到阵列上所点基因cDNA的标记cDNA探针的强度。准确提取基因表达强度值对于进一步的数据分析和对所得结果的解释至关重要。在这里，我们描述了一种用Microsoft Visual Basic开发的新的阵列图像处理软件——ArrayExplorer，它提供了一个用户友好的多窗口界面以及许多自动和手动功能，便于从滤膜阵列图像中可靠、稳健且准确地提取基因强度值。
