Sigdestad C P, Grdina D J
Experientia. 1981 May 15;37(5):501-2. doi: 10.1007/BF01986162.
Tumor cells isolated from a murine fibrosarcoma were grown in primary culture for two days and then separated on a basis of size by velocity sedimentation. Centrifugal elutriation and STAPUT methods were compared to their ability to isolate biophysically unique tumor subpopulations. The isolated cell fractions were assayed for cell number, incorporation of tritiated thymidine and Coulter volume. Both methods were comparable with regard to ability to separate tumor cells on a basis of size. Elutriation had the advantage of speed but required sophisticated equipment. The STAPUT method was less expensive but required somewhat longer times for separation.
从鼠纤维肉瘤中分离出的肿瘤细胞在原代培养中生长两天，然后通过速度沉降根据大小进行分离。比较了离心淘析法和STAPUT法分离生物物理特性独特的肿瘤亚群的能力。对分离出的细胞组分进行细胞数量、氚标记胸腺嘧啶核苷掺入量和库尔特体积的测定。两种方法在根据大小分离肿瘤细胞的能力方面相当。淘析法的优点是速度快，但需要精密设备。STAPUT法成本较低，但分离所需时间稍长。
