Na B K, Lee J S, Shin G C, Shin J M, Lee J Y, Chung J K, Ha D R, Lee J K, Ma S H, Cho H W, Kang C, Kim W J
Department of Virology, National Institute of Health, 122-701, Seoul, South Korea.
Virus Res. 2001 Dec 4;81(1-2):143-9. doi: 10.1016/s0168-1702(01)00346-x.
To characterize the genetic properties of currently circulating measles viruses in Korea, the complete nucleotide sequences of hemagglutinin (H) protein and nucleoprotein (N) genes of Korean viruses were analyzed. The entire genes of H and N were directly amplified by RT-PCR from each clinical specimen and sequenced. Sequence analyses of H and N genes indicated that all Korean viruses had a high degree of homology (>99.8%) when compared with each other. The Korean viruses differed from other wild-type viruses by as much as 6.8% in the H gene and 6.5% in the N gene at the nucleotide level. The deduced amino acid variability was up to 6.4% for the H protein and up to 6.5% for the N protein. Phylogenetic analyses of nucleotide sequences and deduced amino acid sequences of the H and N genes revealed that all Korean viruses were grouped into the clade H1.
为了描述韩国目前流行的麻疹病毒的基因特性，对韩国病毒的血凝素（H）蛋白和核蛋白（N）基因的完整核苷酸序列进行了分析。通过RT-PCR从每个临床标本中直接扩增H和N的完整基因并进行测序。H和N基因的序列分析表明，所有韩国病毒相互比较时具有高度同源性（>99.8%）。在核苷酸水平上，韩国病毒与其他野生型病毒在H基因上的差异高达6.8%，在N基因上的差异高达6.5%。推导的氨基酸变异性对于H蛋白高达6.4%，对于N蛋白高达6.5%。H和N基因的核苷酸序列及推导氨基酸序列的系统发育分析表明，所有韩国病毒都被归入H1分支。
