与共价连接到豇豆花叶病毒基因组的蛋白质相邻的核苷酸序列。使用RNA连接酶进行体外标记后的序列测定。
Nucleotide sequences adjacent to the proteins covalently linked to the cowpea mosaic virus genome. Sequence determination after labelling in vitro using RNA ligase.
作者信息
Stanley J, Van Kammen A
出版信息
Eur J Biochem. 1979 Nov 1;101(1):45-9. doi: 10.1111/j.1432-1033.1979.tb04214.x.
Abstract
The sequences of the first 17 nucleotides of cowpea mosaic virus middle and bottom RNAs adjacent to the covalently-linked proteins have been determined. Sequences of the oligonucleotides, produced by complete T1 RNase digestion, were established after labelling of the 3' termini in vitro using RNA ligase. Both sequences are A/U-rich, the first nine nucleotides being identical.
摘要
已确定豇豆花叶病毒中部和底部RNA与共价连接蛋白相邻的前17个核苷酸的序列。通过完全T1核糖核酸酶消化产生的寡核苷酸序列，在使用RNA连接酶体外标记3'末端后得以确定。两个序列都富含A/U，前九个核苷酸相同。
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