Department of Virus Research, John Innes Institute, Colney Lane, Norwich NR4 7UH, UK.
EMBO J. 1983;2(12):2253-8. doi: 10.1002/j.1460-2075.1983.tb01731.x.
The complete sequence of the bottom component RNA (B RNA) of cowpea mosaic virus (CPMV) has been determined. Restriction enzyme fragments of double-stranded cDNA were cloned in M13 and the sequence of the inserts was determined by a combination of enzymatic and chemical sequencing techniques. Additional sequence information was obtained by primed synthesis on first strand cDNA. The complete sequence deduced is 5889 nucleotides long excluding the 3' poly(A), and contains an open reading frame sufficient to code for a polypeptide of mol. wt. 207 760. The coding region is flanked by a 5' leader sequence of 206 nucleotides and a 3' non-coding region of 82 residues which does not contain a polyadenylation signal.
豇豆花叶病毒（CPMV）底部元件 RNA（B RNA）的完整序列已经确定。双链 cDNA 的限制酶片段被克隆到 M13 中，并通过酶切和化学测序技术组合确定了插入序列的序列。通过对第一链 cDNA 的引物合成获得了额外的序列信息。完整序列扣除 3' 多聚（A）后为 5889 个核苷酸，包含一个开放阅读框，足以编码分子量为 207760 的多肽。编码区侧翼是 206 个核苷酸的 5' 前导序列和 82 个核苷酸的 3' 非编码区，后者不包含多聚腺苷酸化信号。
