Davies J W, Stanley J, Van Kammen A
Nucleic Acids Res. 1979 Sep 25;7(2):493-500. doi: 10.1093/nar/7.2.493.
We have determined the sequence of 80 nucleotides adjacent to 3' poly(A) of both (middle and bottom component) cowpea mosaic virus RNAs, using dideoxynucleotide termination of reverse transcription. Sequence conservation is indicated, there being about 80% homology between the first 65 bases of each RNA. Although both RNAs are polyadenylated, there is no AAUAAA sequence as associated with most known polyadenylated mRNAs of eukaryotes and viruses. However both RNAs are U and A-rich in this region.
我们利用逆转录的双脱氧核苷酸终止法,确定了豇豆花叶病毒RNA(中部和底部组分)3' 多聚腺苷酸附近80个核苷酸的序列。结果表明存在序列保守性,每种RNA的前65个碱基之间约有80%的同源性。虽然两种RNA都进行了多聚腺苷酸化,但不存在与大多数已知真核生物和病毒的多聚腺苷酸化mRNA相关的AAUAAA序列。然而,两种RNA在该区域富含U和A。