Härfast B, Andersson T, Perlmann P
J Immunol. 1975 Jun;114(6):1820-3.
Subpopulations of human lymphocytes were tested for their capacity to kill mumps virus-infected target cells in a 51-chromium release asaay. Using two different cell fractionation techniques, lymphocytes were fractionated into T cell-enriched (primarily T cells) and T cell-depleted (primarily B cells) subpopulations. Filtration of lymphocytes through columns coated with human immunoglobulin and rabbit anti-human-immunoglobulin (Ig-anti-Ig) rendered the resulting T-cell preparation inactive as effector cells against target cells carrying mumps virus antigens. In the second technique, lymphocytes were fractionated by centrifugation into two fractions according to their ability to form spontaneous rosettes with sheep erythrocytes (E). The E-rosette-forming population (primarily T cells) was shown to lack cytotoxic activity against mumps virus-infected target cells. This activity was present in the nonrosetting population. The results suggest that the effector cells involved in this cytotoxic system are of a non-T variety.
采用51铬释放试验检测人淋巴细胞亚群杀伤腮腺炎病毒感染靶细胞的能力。运用两种不同的细胞分级分离技术，将淋巴细胞分级分离成富含T细胞（主要为T细胞）和去除T细胞（主要为B细胞）的亚群。使淋巴细胞通过包被人免疫球蛋白和兔抗人免疫球蛋白（Ig抗Ig）的柱进行过滤，结果得到的T细胞制剂作为针对携带腮腺炎病毒抗原的靶细胞的效应细胞失去活性。在第二种技术中，根据淋巴细胞与绵羊红细胞（E）形成自发玫瑰花结的能力，通过离心将淋巴细胞分成两部分。结果显示，形成E玫瑰花结的细胞群体（主要为T细胞）对腮腺炎病毒感染的靶细胞缺乏细胞毒性活性。这种活性存在于不形成玫瑰花结的细胞群体中。结果提示，参与该细胞毒性系统的效应细胞属于非T细胞类型。
