Paris F, Weinbauer G F, Blüm V, Nieschlag E
Institute of Reproductive Medicine, University of Münster, Germany.
J Steroid Biochem Mol Biol. 1994 Jan;48(1):129-37. doi: 10.1016/0960-0760(94)90259-3.
The androgen receptor (AR) was localized immunohistochemically after different hormonal treatments in the ventral prostate, coagulating gland, seminal vesicle and epididymis of the adult rat. In the untreated controls AR-immunoreactivity was confined to the cell nuclei. One week after castration or treatment with the gonadotropin-releasing hormone antagonist Cetrorelix (150 micrograms/animal per day) a cytoplasmic staining occurred in the epithelial cells of the ventral prostate and in part of the coagulating gland and seminal vesicle. In contrast, the AR remained exclusively in the nuclei in the epididymal epithelium and the glandular smooth muscle layer even after 2 weeks of androgen depletion. Bolus injections of either dihydrotestosterone (1 mg/kg), the antiandrogen flutamide (40 mg/kg), or the novel non-steroidal antiandrogen casodex (40 mg/kg) to androgen-depleted animals eliminated cytoplasmic AR-immunoreactivity and restored the nuclear staining pattern in the ventral prostate. A sustained 2-week treatment with the antiandrogens resulted in a loss of weight in all organs but did not alter the distribution of AR-immunoreactivity. The data show an apparent cytoplasmic/nuclear ligand-dependent translocation of the AR in the ventral prostate, coagulating gland and seminal vesicle but not in the epididymis of the adult rat.
对成年大鼠的腹侧前列腺、凝固腺、精囊和附睾进行不同激素处理后，采用免疫组织化学方法对雄激素受体（AR）进行定位。在未经处理的对照组中，AR免疫反应仅限于细胞核。去势或用促性腺激素释放激素拮抗剂西曲瑞克（每天150微克/只动物）处理1周后，腹侧前列腺、部分凝固腺和精囊的上皮细胞出现细胞质染色。相比之下，即使在雄激素耗竭2周后，AR仍仅存在于附睾上皮和腺性平滑肌层的细胞核中。给雄激素耗竭的动物一次性注射二氢睾酮（1毫克/千克）、抗雄激素氟他胺（40毫克/千克）或新型非甾体抗雄激素比卡鲁胺（40毫克/千克），可消除腹侧前列腺细胞质AR免疫反应，并恢复细胞核染色模式。用抗雄激素持续治疗2周会导致所有器官重量减轻，但不会改变AR免疫反应的分布。数据显示，成年大鼠腹侧前列腺、凝固腺和精囊中AR存在明显的细胞质/细胞核配体依赖性易位，但附睾中不存在。
