Rechner A R, Pannala A S, Rice-Evans C A
Antioxidant Research Centre, Centre of Age-Related Diseases, GKT School of Biomedical Sciences, King's College London, London SE1 9RT.
Free Radic Res. 2001 Aug;35(2):195-202. doi: 10.1080/10715760100300741.
The purpose of this study was to investigate the absorption and metabolism of hydroxycinnamates from artichoke extract by determining the urinary excretion of the conjugates. Ten healthy, non smoking volunteers (5 female, 5 male) were given three capsules containing artichoke extract every 4 h (0, 4, 8 h) following two days of a low-polyphenol diet. One capsule contained 320 mg of artichoke extract equivalent to 34.3 +/- 0.6 mg/g hydroxycinnamates (caffeic acid derivatives) and 5.6 +/- 0.1 mg/g flavonoids. Polyphenols and phenolic acids present in the artichoke extract were not detected in the urine either as conjugates or aglycones. However, ferulic, isoferulic, dihydroferulic and vanillic acid were identified as major metabolites after beta-glucuronidase treatment of urine. The amount excreted as well as the ratio to that of creatinine, a biomarker for the general excretion rate, increased significantly on the study day compared to the pre-supplementation day. Thus, the caffeic acid esters found in the artichoke extract capsule are absorbed, metabolised and excreted as methylated phenolic acids such as ferulic, isoferulic, dihydroferulic and vanillic acid.
本研究的目的是通过测定结合物的尿排泄量来研究朝鲜蓟提取物中羟基肉桂酸的吸收和代谢情况。在进行了两天的低多酚饮食后，10名健康的非吸烟志愿者（5名女性，5名男性）每4小时（0、4、8小时）服用三粒含有朝鲜蓟提取物的胶囊。一粒胶囊含有320毫克朝鲜蓟提取物，相当于34.3±0.6毫克/克羟基肉桂酸（咖啡酸衍生物）和5.6±0.1毫克/克黄酮类化合物。在尿液中未检测到朝鲜蓟提取物中存在的多酚和酚酸的结合物或苷元形式。然而，在对尿液进行β-葡萄糖醛酸酶处理后，阿魏酸、异阿魏酸、二氢阿魏酸和香草酸被鉴定为主要代谢产物。与补充前一天相比，研究当天作为代谢产物排泄的量以及与肌酐（一般排泄率的生物标志物）的比值均显著增加。因此，朝鲜蓟提取物胶囊中发现的咖啡酸酯会被吸收、代谢并以甲基化酚酸如阿魏酸、异阿魏酸、二氢阿魏酸和香草酸的形式排泄。
