Department of Molecular Drug Evaluation, National Organization for Drug Control & Research (NODCAR), Giza, Egypt.
Environ Sci Pollut Res Int. 2019 Nov;26(31):31675-31684. doi: 10.1007/s11356-019-06099-6. Epub 2019 Sep 4.
Oxidative stress is an imbalance between free radicals and antioxidants which leads to reactive oxygen species (ROS) production in cells. Reactive oxygen species contains oxygen radicals that easily react with other molecules in the biological system. For decades, lead acetate (Pb(CHO2)) is used as an additive for many widely used chemical products such as insecticides, hair dyes, and cosmetics; however, contact with lead acetate may irritate skin, eyes, and mucous membranes.In the present study, the antioxidant and anti-inflammatory effect of using ferulic acid to inhibit lead acetate-induced toxicity in rats is investigated. Lead acetate was orally given at a dose of 20 mg/kg body weight for 10 days, either alone or with ferulic acid at dose 25 mg/kg. Serum luteinizing hormone (LH), total testosterone, and follicle-stimulating hormone (FSH) levels were measured. Also, reactive oxygen species (ROS), lipid peroxidation (LPO), total antioxidant capacity (TAC), and catalase (CAT) activities were determined. In addition, histopathological changes of testes and kidney were examined. Results showed that administration of lead acetate induced oxidative stress through attenuation of luteinizing hormone, total testosterone, and follicle-stimulating hormone levels in serum. Moreover, the kidney and testes of lead acetate-treated animals exhibited elevation of ROS level, lipid peroxide levels, as well as lysosomal enzyme activity such acid phosphatase and N-acetyl-β-glucosminidase. DNA fragmentation and histological changes were also observed in lead acetate-treated group. In contrast, ferulic acid treatment reduced the deleterious effects induced by lead acetate in both testes and kidney tissues. These results illustrated that ferulic acid has a protective action against toxicity caused by lead acetate in rats. In conclusions, ferulic acid may have future therapeutic relevance in the prevention of lead acetate-induced testicular and renal toxicity in rats.
氧化应激是自由基和抗氧化剂之间的失衡，导致细胞内活性氧（ROS）的产生。活性氧含有容易与生物系统中其他分子反应的氧自由基。几十年来，醋酸铅（Pb（CHO2））一直被用作许多广泛使用的化学产品的添加剂，如杀虫剂、染发剂和化妆品；然而，接触醋酸铅可能会刺激皮肤、眼睛和粘膜。在本研究中，研究了使用阿魏酸抑制醋酸铅诱导的大鼠毒性的抗氧化和抗炎作用。醋酸铅以 20mg/kg 体重的剂量口服给予 10 天，单独或与 25mg/kg 的阿魏酸一起给予。测量血清黄体生成素（LH）、总睾酮和卵泡刺激素（FSH）水平。还测定了活性氧（ROS）、脂质过氧化（LPO）、总抗氧化能力（TAC）和过氧化氢酶（CAT）活性。此外，检查了睾丸和肾脏的组织病理学变化。结果表明，醋酸铅的给药通过降低血清中黄体生成素、总睾酮和卵泡刺激素水平引起氧化应激。此外，醋酸铅处理动物的肾脏和睾丸表现出 ROS 水平、脂质过氧化物水平以及酸性磷酸酶和 N-乙酰-β-葡萄糖苷酶等溶酶体酶活性的升高。在醋酸铅处理组中还观察到 DNA 片段化和组织学变化。相比之下，阿魏酸处理减轻了醋酸铅对睾丸和肾脏组织的有害影响。这些结果表明，阿魏酸对大鼠醋酸铅引起的毒性具有保护作用。总之，阿魏酸可能在预防大鼠醋酸铅诱导的睾丸和肾毒性方面具有未来的治疗相关性。
