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[在琼脂培养基上长期保存的放线菌培养物的活力研究]

[Viability study of actinomycete cultures preserved for a long time on agar media].

作者信息

Semenov S M

出版信息

Antibiotiki. 1978 May;23(5):391-4.

PMID:655683
Abstract

Viability of 254 actinomycetous strains stored for 1 to 6 years on agarized media a temperature of 4-10 degrees C without passages was studied. The cultures included 204 strain of Actinomyces, 26 strains of Mycobacterium, 2 strains of Streptosporangium, 1 strain of Intrasporangium, 2 strains of Micropolyspora and 5 strains of Thermoactinomyces. Most of the cultures, i.e. 94.55 per cent stored for 6 years remained vialbe after the this period. With an increase in the storage period from 1 to 4 years the number of the viable cultures stored for such periods of time gradually decreased from 95.28 to 73.11 per cent. The representatives of Micromonospora and Streptosporangium, some strains of Proactinomyces and the cultures of Actinomyces forming whorl sporophores were the least stable when stored for a period up to 1 year. It was noted that viability of different strains within the same species was not the same in storage.

摘要

研究了254株放线菌在琼脂培养基上于4 - 10摄氏度温度下保存1至6年且未传代的存活情况。这些培养物包括204株放线菌、26株分枝杆菌、2株链孢囊菌、1株内孢囊菌、2株小多孢菌和5株嗜热放线菌。大部分培养物,即94.55%保存6年的培养物在此期间后仍存活。随着保存期从1年增加到4年,在此时间段内存活培养物的数量从95.28%逐渐减少到73.11%。小单孢菌和链孢囊菌的代表菌株、一些原放线菌菌株以及形成轮状分生孢子的放线菌培养物在保存长达1年时最不稳定。值得注意的是,同一物种内不同菌株在保存时的存活能力并不相同。

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