Lamas M C, Bregni C, Daquino M, Degrossi J, Firenstein R
Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Argentina.
Drug Dev Ind Pharm. 2001 Sep;27(8):825-9. doi: 10.1081/ddc-100107246.
Microspheres of Bacillus subtilis were prepared using sodium alginate. Some typical properties of microencapsulated systems, such as microorganism content, particle size, and germination time, were studied. Calcium alginate microspheres were obtained by the emulsification method, dripping into a solution of calcium salt. The conditions of the preparation steps were very soft to produce calcium alginate microspheres containing cells with no apparent changes in general biological properties. The hydrogel matrix provides protection without preventing communication with the surrounding medium.
使用海藻酸钠制备枯草芽孢杆菌微球。研究了微囊化系统的一些典型特性，如微生物含量、粒径和萌发时间。通过乳化法将其滴入钙盐溶液中获得海藻酸钙微球。制备步骤的条件非常温和，以生产含有细胞的海藻酸钙微球，其一般生物学特性没有明显变化。水凝胶基质提供保护，同时不阻止与周围介质的交流。
