Chernitsky E A, Senkovich O A, Rozin V V
Institute of Photobiology, National Academy of Sciences of Belarus, Minsk, Republic of Belarus.
Membr Cell Biol. 2001 Jul;14(5):629-36.
The kinetic and concentration dependences of erythrocyte vesiculation and hemolysis induced by sodium dodecyl sulfate were studied. The similarity of the slopes of the dose dependence of the SDS-induced vesiculation and slow hemolysis rates in the double logarithmic coordinates suggested a close relation between the processes of vesiculation and pore formation for slow hemolysis by the detergent. Further evidence of the competitive nature of the detergent-induced vesiculation and fast hemolysis by sodium dodecyl sulfate was obtained. The phenomenon of partial hemolysis proceeding at a rate comparable to that of cell vesiculation is explained in terms of the competition between hemolysis and vesiculation, without resorting to erythrocyte heterogeneity. New vesicular-competitive hemolysis is described. Based on it, the action of different hemolysis-inducing agents is analysed.
研究了十二烷基硫酸钠诱导红细胞形成囊泡和溶血的动力学及浓度依赖性。在双对数坐标中，十二烷基硫酸钠诱导囊泡形成和缓慢溶血速率的剂量依赖性斜率相似，这表明去污剂诱导的囊泡形成过程与缓慢溶血的孔形成过程之间存在密切关系。进一步获得了十二烷基硫酸钠诱导囊泡形成和快速溶血具有竞争性的证据。部分溶血以与细胞形成囊泡速率相当的速度进行这一现象，可通过溶血与囊泡形成之间的竞争来解释，而无需考虑红细胞的异质性。描述了新的囊泡竞争性溶血。基于此，分析了不同溶血诱导剂的作用。
