Chernitsky E A, Senkovich O A
Institute of Photobiology, Belorussian Academy of Sciences, Minsk, Republic of Belarus.
Membr Cell Biol. 1997;11(4):475-85.
The numbers of Triton X-100 and sodium dodecyl sulfate molecules required to form respective pores were estimated from the relationship between the detergent concentrations and the rates of fast and slow hemolysis components. It has been found that the slow hemolysis component evoked by Triton X-100 is related to the existence of two different pores. It is shown that the fast hemolysis component induced by sodium dodecyl sulfate is associated with the modification of phosphatidylcholine which determines the break in the Arrhenius plots of the hemolysis rate within the range of 20 degrees C. The shape of hemolysis kinetic curves and the dependence of hemolytic parameters on the detergent concentration and temperature are discussed based on the concept of hemolysis caused by the formation of pores in various membrane lipid regions and by releasing vesicles from erythrocytes.
根据去污剂浓度与快速和慢速溶血成分速率之间的关系，估算了形成各自孔隙所需的 Triton X-100 和十二烷基硫酸钠分子的数量。已发现 Triton X-100 引起的慢速溶血成分与两种不同孔隙的存在有关。结果表明，十二烷基硫酸钠诱导的快速溶血成分与磷脂酰胆碱的修饰有关，这决定了在 20 摄氏度范围内溶血速率的阿累尼乌斯图中的断点。基于在各种膜脂质区域形成孔隙以及从红细胞释放囊泡导致溶血的概念，讨论了溶血动力学曲线的形状以及溶血参数对去污剂浓度和温度的依赖性。
