Hagan S N, Tietjen W H
J Assoc Off Anal Chem. 1975 May;58(3):620-1.
A thin layer chromatographic cleanup development with benzene-hexane (3+1) effectively removed lipids and some contaminants from mixtures of mycotoxins in corn oil, olive oil, peanut oil, soybean oil, and seed extracts. A second development in the same direction as the first, using toluene-ethyl acetate-formic acid (6+3+1) or benzene-acetic acid (9+1), separated the mycotoxins. Satisfactory separation was achieved for commercial oils spiked with sterigmatocystin, zearalenone, ochratoxins A, B, and C, and aflatoxins B1, B2, G1, and G2. This technique permits detection of 5 ppb aflatoxin B1 in corn.
采用苯 - 己烷（3 + 1）进行薄层色谱净化展开，能有效去除玉米油、橄榄油、花生油、大豆油及种子提取物中霉菌毒素混合物中的脂质和一些污染物。沿与第一次相同的方向进行第二次展开，使用甲苯 - 乙酸乙酯 - 甲酸（6 + 3 + 1）或苯 - 乙酸（9 + 1），可分离霉菌毒素。对于添加了柄曲霉素、玉米赤霉烯酮、赭曲霉毒素A、B和C以及黄曲霉毒素B1、B2、G1和G2的市售油，实现了令人满意的分离。该技术能够检测玉米中5 ppb的黄曲霉毒素B1。
