Wilson D M, Tabor W H, Trucksess M W
J Assoc Off Anal Chem. 1976 Jan;59(1):125-7.
A modification of the official method for ochratoxins and a screening method for zearalenone, aflatoxin, and ochratoxin is described and expanded to include citrinin and penicillic acid. The method uses 0.5N phosphoric acidchloroform (1+10) in the initial extraction; the extract is divided and eluted from 2 columns to provide a quantitative thin layer chromatographic (TLC) method for aflatoxin and ochratoxin in corn and dried beans. Aflatoxin and zearalenone are eluted from one column and ochratoxin, penicillic acid, and citrinin from the other. Ochratoxin A recoveries are low (50%) in peanuts. Zearalenone, penicillic acid, and citrinin were qualitatively recovered from corn and beans; zearalenone and penicillic acid were recovered from peanuts but citrinin was not. Several TLC solvents were used to separate interferences.
本文描述并扩展了一种针对赭曲霉毒素的官方方法以及一种针对玉米赤霉烯酮、黄曲霉毒素和赭曲霉毒素的筛选方法，使其包括桔青霉素和青霉酸。该方法在初始提取中使用0.5N磷酸 - 氯仿（1 + 10）；提取物被分开并从两根柱上洗脱，以提供一种用于玉米和干豆中黄曲霉毒素和赭曲霉毒素的定量薄层色谱（TLC）方法。黄曲霉毒素和玉米赤霉烯酮从一根柱上洗脱，赭曲霉毒素、青霉酸和桔青霉素从另一根柱上洗脱。花生中赭曲霉毒素A的回收率较低（50%）。玉米赤霉烯酮、青霉酸和桔青霉素在玉米和豆类中得到定性回收；玉米赤霉烯酮和青霉酸在花生中得到回收，但桔青霉素未回收。使用了几种TLC溶剂来分离干扰物。
