Lauren D R, Jensen D J, Douglas J A, Follett J M
Horticulture and Food Research Institute of New Zealand, Ruakura Research Centre, Private Bag 3123, Hamilton, New Zealand.
Phytochem Anal. 2001 Sep-Oct;12(5):332-5. doi: 10.1002/pca.597.
A reliable and efficient method suitable for the selective analysis of the glycyrrhizin content of fresh and dried roots of Glycyrrhiza species and of root extracts is described. The method was optimised for recovery and analysis of glycyrrhizin after conversion to its aglycone, 18 beta-glycyrrhetinic acid. The reversed-phase HPLC system was developed using a sterically protected C8 column that allowed UV detection of the aglycone at 254 mm without interference from co-occurring components. The average recovery through the method was determined at 83%, with a range of 65-99%. Analysis of 150 dried root samples gave levels of glycyrrhizin consistent with earlier reported values. A comparison of post-harvest treatment conditions. showed that the drying of root samples at up to 65 degrees C did not result in any measurable reduction in glycyrrhizin.
本文描述了一种可靠且高效的方法，适用于选择性分析甘草属植物新鲜和干燥根及根提取物中的甘草酸含量。该方法针对将甘草酸转化为其苷元18β-甘草次酸后的回收率和分析进行了优化。反相高效液相色谱系统采用空间保护的C8柱开发，可在254nm处对苷元进行紫外检测，不受共存成分干扰。该方法的平均回收率为83%，范围为65%-99%。对150个干燥根样品的分析得出的甘草酸水平与早期报告的值一致。收获后处理条件的比较表明，在高达65℃的温度下干燥根样品不会导致甘草酸有任何可测量的减少。
