采用等度洗脱的高效液相色谱法同时分析甘草根及糖果制品中的甘草酸和甘草次酸。
Simultaneous HPLC analysis, with isocratic elution, of glycyrrhizin and glycyrrhetic acid in liquorice roots and confectionery products.
作者信息
Sabbioni Cesare, Ferranti Anna, Bugamelli Francesca, Forti Giorgio Cantelli, Raggi Maria Augusta
机构信息
Department of Pharmaceutical Sciences, Faculty of Pharmacy, Alma Mater Studiorum, University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy.
出版信息
Phytochem Anal. 2006 Jan-Feb;17(1):25-31. doi: 10.1002/pca.877.
Abstract
Glycyrrhizin (1), the main active principle of Glycyrrhiza glabra (liquorice) roots, is extensively used in herbal medicines, in pharmaceutical preparations and confectionery products. A feasible and reliable method which allows the simultaneous analysis of 1 and its aglycone, 18beta-glycyrrhetic acid (2), by means of an isocratic HPLC procedure is described. The system uses a C8 column as the stationary phase, and a mixture of acetonitrile, methanol, water and glacial acetic acid as the mobile phase. Good linearity was found in the concentration ranges 1-50 and 0.05-2.50 microg/mL for 1 and 2, respectively. A simple and rapid sample pre-treatment, based on the extraction of the two analytes with a mixture of water and ethanol, was developed for the examination of liquorice confectionery products and root samples. The HPLC method was shown to be appropriate, in terms of precision and feasibility, for the quality control of the analytes in these matrices.
摘要
甘草甜素（1）是光果甘草根的主要活性成分，广泛应用于草药、药物制剂和糖果产品中。本文描述了一种可行且可靠的方法，该方法可通过等度高效液相色谱法同时分析1及其苷元18β-甘草次酸（2）。该系统使用C8柱作为固定相，乙腈、甲醇、水和冰醋酸的混合物作为流动相。1和2的浓度范围分别为1 - 50和0.05 - 2.50μg/mL时，线性关系良好。基于用水和乙醇混合物萃取两种分析物，开发了一种简单快速的样品预处理方法，用于检测甘草糖果产品和根样品。就精密度和可行性而言，高效液相色谱法适用于这些基质中分析物的质量控制。
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